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Creating supported plasma membrane bilayers using acoustic pressure

Erdinc Sezgin, Dario Carugo, Ilya Levental, Eleanor Stride, Christian Eggeling
doi: https://doi.org/10.1101/2020.01.20.912840
Erdinc Sezgin
1MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, UK
2Science for Life Laboratory, Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden
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  • For correspondence: erdinc.sezgin@ki.se christian.eggeling@uni-jena.de
Dario Carugo
3Bioengineering Sciences Research Groups, Faculty of Engineering and Physical Sciences, Institute for Life Sciences (IfLS), University of Southampton, Southampton, UK
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Ilya Levental
4McGovern Medical School, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, USA
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Eleanor Stride
5Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK
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Christian Eggeling
1MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS, Oxford, UK
6Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Max-Wien Platz 4, 07743 Jena, Germany
7Leibniz Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745 Jena, Germany
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  • For correspondence: erdinc.sezgin@ki.se christian.eggeling@uni-jena.de
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Abstract

Model membrane systems are essential tools for biology, enabling study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between native cellular plasma and artificial membranes. Certain applications, however, require planar membrane surfaces. Here, we report a novel approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using an ultrasonic pressure field generated within an acoustofluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems will be useful for many applications requiring detailed characterization of plasma membrane dynamics.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted January 20, 2020.
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Creating supported plasma membrane bilayers using acoustic pressure
Erdinc Sezgin, Dario Carugo, Ilya Levental, Eleanor Stride, Christian Eggeling
bioRxiv 2020.01.20.912840; doi: https://doi.org/10.1101/2020.01.20.912840
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Creating supported plasma membrane bilayers using acoustic pressure
Erdinc Sezgin, Dario Carugo, Ilya Levental, Eleanor Stride, Christian Eggeling
bioRxiv 2020.01.20.912840; doi: https://doi.org/10.1101/2020.01.20.912840

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