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Drosophila dTBCE recruits tubulin around chromatin to promote mitotic spindle assembly

Mathieu Métivier, View ORCID ProfileEmmanuel Gallaud, Aude Pascal, View ORCID ProfileJean-Philippe Gagné, View ORCID ProfileGuy G. Poirier, View ORCID ProfileDenis Chrétien, View ORCID ProfileRomain Gibeaux, Laurent Richard-Parpaillon, View ORCID ProfileChristelle Benaud, View ORCID ProfileRégis Giet
doi: https://doi.org/10.1101/2020.01.21.912428
Mathieu Métivier
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Emmanuel Gallaud
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Aude Pascal
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Jean-Philippe Gagné
2Centre de Recherche du Centre Hospitalier Universitaire de Québec – Pavillon CHUL, Université Laval, Québec, QC, Canada
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Guy G. Poirier
2Centre de Recherche du Centre Hospitalier Universitaire de Québec – Pavillon CHUL, Université Laval, Québec, QC, Canada
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Denis Chrétien
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Romain Gibeaux
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Laurent Richard-Parpaillon
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Christelle Benaud
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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Régis Giet
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, F-35000 Rennes, France
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  • For correspondence: regis.giet@univ-rennes1.fr
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Abstract

Proper assembly of mitotic spindles requires microtubule nucleation at centrosomes but also around chromatin. In this study, we reveal a novel mechanism by which an enrichment of tubulin in the nuclear space following nuclear envelope breakdown promotes nucleation of spindle microtubules. This event mediated by the tubulin-specific chaperone dTBCE, depends on its tubulin binding CAP-Gly motif and is regulated by Ran. Live imaging, proteomic and biochemical analyses suggest that dTBCE is enriched in the nucleus at nuclear envelope breakdown and interacts with nuclear pore proteins and the Ran machinery to create an environment that facilitates subsequent tubulin enrichment. We propose that dTBCE-dependent increase in tubulin concentration in the nuclear space is an important mechanism for microtubule nucleation in organisms where compartmentalization prevents free diffusion of tubulin.

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Posted January 21, 2020.
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Drosophila dTBCE recruits tubulin around chromatin to promote mitotic spindle assembly
Mathieu Métivier, Emmanuel Gallaud, Aude Pascal, Jean-Philippe Gagné, Guy G. Poirier, Denis Chrétien, Romain Gibeaux, Laurent Richard-Parpaillon, Christelle Benaud, Régis Giet
bioRxiv 2020.01.21.912428; doi: https://doi.org/10.1101/2020.01.21.912428
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Drosophila dTBCE recruits tubulin around chromatin to promote mitotic spindle assembly
Mathieu Métivier, Emmanuel Gallaud, Aude Pascal, Jean-Philippe Gagné, Guy G. Poirier, Denis Chrétien, Romain Gibeaux, Laurent Richard-Parpaillon, Christelle Benaud, Régis Giet
bioRxiv 2020.01.21.912428; doi: https://doi.org/10.1101/2020.01.21.912428

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