Abstract
Background Gut microbiota, mainly characterized by fecal bacterial compositions, affects human immune system and pathophysiological development. Our aim was to measure the quantitative differences of fecal bacterial compositions between osteoporotic patients and healthy subjects, and to identify novel bacterial taxa that speculate the incidence of osteoporosis in female.
Method We recruited 104 female subjects, including 45 osteoporotic individuals and 59 healthy control. Fecal samples were collected for further analysis by 16S rRNA quantitative arrays and bioinformatics analysis.
Results Analyses of α- and β-diversity demonstrated that the diversity and composition of fecal bacterial compositions were both significant different in osteoporosis group, as compared with healthy group. Multiple bacterial genera were significantly increased (e.g., Roseburia and Bacteroides) or decreased (e.g., Streptococcus and Dorea) in the osteoporotic cases. Furthermore, the osteoporosiscould be efficiently determined by the random forest model based on differential taxa (area under ROC curve = 0.93).
Conclusion There were obvious different fecal microbial characteristics between female osteoporosis and healthy subjects. These findings provided evidence for understanding the host-gut microbiota interplay in female osteoporosis, and supported clinical applications of gut microbiota analysis for female osteoporosis diagnosis
Footnotes
Cuifeng Zhu, Jianguo Liu and Yong Pan contributed equally to this work. Author order was determined on the basis of seniority. (CuiFeng Zhu, Department director of Dept of Clinical Nutrition, chief physician, master tutor of Shenzhen hospital of southern medical university. Post doctor fellows of Dept of endocrinology of university of Hong Kong & The State Key laboratory of Pharmaceutical Biotechnology. E-mail: 1794371266{at}qq.com (Contributions to this study: chief physician of Nutrition department and post doctorate, responsible for the design and implementation of this project, data collation and paper writing); JianGuo Liu, Associate chief physician, Department director of Dept of Health management of Shenzhen hospital of southern medical University. E-mail: JgLiu123{at}163.com(Contribution to this study: general practitioner at health management center, responsible for case collection and data collation); Pan Yong, Post doctor fellows of Dept of endocrinology of university of Hong Kong & The State Key laboratory of Pharmaceutical Biotechnology. E-mail: panicy0322{at}hotmail.com. (Contributions to this study: post doctorate, responsible for data collation and thesis writing guidance of this project)
Other author: Ye Fan: School of Stomatology and Medicine, Foshan University, Foshan, China, 528000, E-mail: lhfanye{at}126.com. Tele Phone:13560735015(Contribution to this study: visiting scholar, responsible for the data processing of intestinal microflora gene polymorphism test results)
Leigang Jin: Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong & The State Key laboratory of Pharmaceutical Biotechnology, Hong Kong, China. E-mail: jinlg520{at}hku.hk. (Contributions to this study:doctor student, responsible for the early data processing of intestinal microflora gene polymorphism)
Yan Liu: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000, E-mail: 853028990{at}qq.com, 13570469574 (Contribution to this study:Nutrition department attending physician, in charge of the medical history of selected patients, menstrual history, life history and other data survey)
Zhentian Zhang: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail: 13365854123{at}163.com, 13544154119. (Contribution to this study: a nutritionist was responsible for the investigation of intestinal mucosal barrier function in selected patients with medical history and other data)
Yu Gan: The Third Affiliatd Hospital of Guangzhou Medical University, E-mail: cf-zhu{at}163.com. (Contribution to this study: biomedical engineer, responsible for the analysis and processing of intestinal flora structure and gene polymorphism detection data)
Wei Tang: Yearth Biotechnology Co. Ltd., Changsha, China, 410000, E-mail: tangwei{at}yearth.cn, 0731-88709258. (Contribution to this study:biomedical engineer, responsible for the extraction of fecal intestinal flora RNA and reverse transcription of DNA)
Jinhuan Li: Yearth Biotechnology Co. Ltd., Changsha, China, 410000, E-mail: lijinhuan{at}yearth.cn. 0731-88709258. (Contribution to this study:biomedical engineer, responsible for the extraction of fecal intestinal flora RNA and reverse transcription of DNA)
Zhuang Xiong: Yearth Biotechnology Co. Ltd., Changsha, China, 410000, E-mail: xiongzhuang{at}yearth.cn. 0731-88709258. (Contribution to this study: biomedical engineer, responsible for the detection and analysis report of fecal intestinal flora gene polymorphism)
Genming Xu: Yearth Biotechnology Co. Ltd., Changsha, China, 410000, E-mail: xugenming{at}yearth.cn. 0731-88709258. (Contribution to this study: biomedical engineer, responsible for the detection and analysis report of fecal intestinal flora gene polymorphism)
Xiuping Lin: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail: 895819039{at}qq.com, 13500053960. (Contribution to this study: head nurse at health management center, responsible for case collection and collection of serum and stool samples from perimenopausal women)
Yuan Zhang: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail: 417053629{at}qq.com, 15118092923. (Contributions to this study: Health management Centre general practitioner, perimenopausal female case collection and collection of serum and stool samples)
JinChuan Cai: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail: e-mail:13600158985{at}163.com, 13600158985. (Contributions to this study: Technician at the health management center, bone density measurement.)
Muxiu Yang: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail:421643681{at}qq.com, 13823236331. (Contributions to this study:: Biomedical engineer, responsible for bone density analysis in this study)
Leijun Zhang: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail: 228802383{at}qq.com, 13802278833. (Contributions to this study:Orthopedic surgeon at health management center, case collection)
Liehui Xiao: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong province, 518000. E-mail: 516672562{at}qq.com, 13189097298. Contributions to this study: integrated medicine physician, case collection)