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C. elegans establishes germline versus soma by balancing inherited histone methylation

View ORCID ProfileBrandon S. Carpenter, View ORCID ProfileTeresa W. Lee, Caroline F. Plott, Jovan S. Brockett, Dexter A. Myrick, View ORCID ProfileDavid J. Katz
doi: https://doi.org/10.1101/2020.01.22.914580
Brandon S. Carpenter
1Department of Cell Biology, Emory University School of Medicine, Atlanta GA 30322, USA
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Teresa W. Lee
1Department of Cell Biology, Emory University School of Medicine, Atlanta GA 30322, USA
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Caroline F. Plott
2Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
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Jovan S. Brockett
3Department of Biology, Oglethorpe University, Atlanta GA 30319, USA
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Dexter A. Myrick
1Department of Cell Biology, Emory University School of Medicine, Atlanta GA 30322, USA
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David J. Katz
1Department of Cell Biology, Emory University School of Medicine, Atlanta GA 30322, USA
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  • For correspondence: djkatz@emory.edu
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ABSTRACT

Embryos undergo extensive reprogramming at fertilization to prevent the inappropriate inheritance of histone methylation. In C. elegans, this reprogramming is mediated by the H3K4me2 demethylase, SPR-5, and the H3K9 methyltransferase, MET-2. In contrast to this reprogramming, the H3K36 methyltransferase, MES-4, maintains H3K36me2/3 at germline genes between generations to help re-establish the germline. To determine whether the MES-4 germline inheritance system antagonizes spr-5; met-2 reprogramming, we examined the interaction between these two systems. We find that the developmental delay of spr-5; met-2 mutant progeny is associated with ectopic H3K36me2/3 and the ectopic expression of MES-4 targeted germline genes in somatic tissues. Furthermore, the developmental delay is dependent upon MES-4 and the H3K4 methyltransferase, SET-2. We propose that the MES-4 inheritance system prevents critical germline genes from being repressed by maternal spr-5; met-2 reprogramming. Thus, the balance of inherited histone modifications is necessary to distinguish germline versus soma and prevent developmental delay.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE143839

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 29, 2020.
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C. elegans establishes germline versus soma by balancing inherited histone methylation
Brandon S. Carpenter, Teresa W. Lee, Caroline F. Plott, Jovan S. Brockett, Dexter A. Myrick, David J. Katz
bioRxiv 2020.01.22.914580; doi: https://doi.org/10.1101/2020.01.22.914580
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C. elegans establishes germline versus soma by balancing inherited histone methylation
Brandon S. Carpenter, Teresa W. Lee, Caroline F. Plott, Jovan S. Brockett, Dexter A. Myrick, David J. Katz
bioRxiv 2020.01.22.914580; doi: https://doi.org/10.1101/2020.01.22.914580

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