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A standardized head-fixation system for performing large-scale, in-vivo physiological recordings in mice

PA Groblewski, D Sullivan, J Lecoq, SEJ de Vries, S Caldejon, Q L’Heureux, T Keenan, K Roll, C Slaughterback, A Williford, C Farrell
doi: https://doi.org/10.1101/2020.01.22.916007
PA Groblewski
Allen Institute for Brain Science, Seattle, WA 98103, USA
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  • For correspondence: peterg@alleninstitute.org
D Sullivan
Allen Institute for Brain Science, Seattle, WA 98103, USA
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J Lecoq
Allen Institute for Brain Science, Seattle, WA 98103, USA
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SEJ de Vries
Allen Institute for Brain Science, Seattle, WA 98103, USA
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S Caldejon
Allen Institute for Brain Science, Seattle, WA 98103, USA
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Q L’Heureux
Allen Institute for Brain Science, Seattle, WA 98103, USA
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T Keenan
Amazon Logistics, Bellevue, WA 98004, USA
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K Roll
Allen Institute for Brain Science, Seattle, WA 98103, USA
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C Slaughterback
Allen Institute for Brain Science, Seattle, WA 98103, USA
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A Williford
Allen Institute for Brain Science, Seattle, WA 98103, USA
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C Farrell
Allen Institute for Brain Science, Seattle, WA 98103, USA
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ABSTRACT

The Allen Institute recently built a set of high-throughput experimental pipelines to collect comprehensive in vivo surveys of physiological activity in the visual cortex of awake, head-fixed mice. Developing these large-scale, industrial-like pipelines posed many scientific, operational, and engineering challenges. Here we describe our strategies for collecting standardized, multi-modal in vivo datasets across many instrumental platforms over long periods of time. Our goal of creating a cross-platform reference space to which all pipeline datasets were mapped required development of 1) a robust headframe, 2) a reproducible clamping system, and 3) data-collection systems that are built, and maintained, around precise alignment with a reference artifact. When paired with our pipeline clamping system, the Allen Brain Observatory headframe exceeded deflection and reproducibility requirements. All headframe, surgical tooling, and clamp design documentation have been made freely available as an open resource to the scientific community.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 23, 2020.
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A standardized head-fixation system for performing large-scale, in-vivo physiological recordings in mice
PA Groblewski, D Sullivan, J Lecoq, SEJ de Vries, S Caldejon, Q L’Heureux, T Keenan, K Roll, C Slaughterback, A Williford, C Farrell
bioRxiv 2020.01.22.916007; doi: https://doi.org/10.1101/2020.01.22.916007
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A standardized head-fixation system for performing large-scale, in-vivo physiological recordings in mice
PA Groblewski, D Sullivan, J Lecoq, SEJ de Vries, S Caldejon, Q L’Heureux, T Keenan, K Roll, C Slaughterback, A Williford, C Farrell
bioRxiv 2020.01.22.916007; doi: https://doi.org/10.1101/2020.01.22.916007

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