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High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation

View ORCID ProfileMateusz Legut, View ORCID ProfileZharko Daniloski, Xinhe Xue, Dayna McKenzie, Xinyi Guo, View ORCID ProfileHans-Hermann Wessels, View ORCID ProfileNeville E. Sanjana
doi: https://doi.org/10.1101/2020.01.22.916064
Mateusz Legut
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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  • ORCID record for Mateusz Legut
Zharko Daniloski
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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Xinhe Xue
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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Dayna McKenzie
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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Xinyi Guo
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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Hans-Hermann Wessels
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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  • ORCID record for Hans-Hermann Wessels
Neville E. Sanjana
1New York Genome Center, New York, NY, USA
2Department of Biology, New York University, New York, NY, USA
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  • ORCID record for Neville E. Sanjana
  • For correspondence: neville@sanjanalab.org
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Article Information

doi 
https://doi.org/10.1101/2020.01.22.916064
History 
  • January 23, 2020.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Mateusz Legut1,2,3,
  2. Zharko Daniloski1,2,3,
  3. Xinhe Xue1,2,
  4. Dayna McKenzie1,2,
  5. Xinyi Guo1,2,
  6. Hans-Hermann Wessels1,2 and
  7. Neville E. Sanjana1,2,*
  1. 1New York Genome Center, New York, NY, USA
  2. 2Department of Biology, New York University, New York, NY, USA
  1. ↵*E-mail: neville{at}sanjanalab.org
  1. ↵3 These authors contributed equally

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Posted January 23, 2020.
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High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation
Mateusz Legut, Zharko Daniloski, Xinhe Xue, Dayna McKenzie, Xinyi Guo, Hans-Hermann Wessels, Neville E. Sanjana
bioRxiv 2020.01.22.916064; doi: https://doi.org/10.1101/2020.01.22.916064
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High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation
Mateusz Legut, Zharko Daniloski, Xinhe Xue, Dayna McKenzie, Xinyi Guo, Hans-Hermann Wessels, Neville E. Sanjana
bioRxiv 2020.01.22.916064; doi: https://doi.org/10.1101/2020.01.22.916064

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