Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A Grain of Salt

Kelly Houston, Jiaen Qiu, View ORCID ProfileStefanie Wege, Maria Hrmova, Helena Oakey, Yue Qu, Pauline Smith, Apriadi Situmorang, Malcolm Macaulay, Paulina Flis, Micha Bayer, View ORCID ProfileStuart Roy, Claire Halpin, Joanne Russell, Caitlin Byrt, View ORCID ProfileMatt Gilliham, David E. Salt, Robbie Waugh
doi: https://doi.org/10.1101/2020.01.23.916742
Kelly Houston
1Cell and Molecular Sciences, The James Hutton Institute, Errol Road Invergowrie, Dundee, DD2 5DA, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiaen Qiu
2ARC Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stefanie Wege
2ARC Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stefanie Wege
Maria Hrmova
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
4School of Life Sciences, Huaiyin Normal University, Huaian 223300, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helena Oakey
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yue Qu
2ARC Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pauline Smith
1Cell and Molecular Sciences, The James Hutton Institute, Errol Road Invergowrie, Dundee, DD2 5DA, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Apriadi Situmorang
2ARC Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Malcolm Macaulay
1Cell and Molecular Sciences, The James Hutton Institute, Errol Road Invergowrie, Dundee, DD2 5DA, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paulina Flis
5Future Food Beacon of Excellence and the School of Biosciences, University of Nottingham, Nottingham NG7 2RD, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Micha Bayer
1Cell and Molecular Sciences, The James Hutton Institute, Errol Road Invergowrie, Dundee, DD2 5DA, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stuart Roy
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
6ARC Industrial Transformation Research Hub for Wheat in a Hot Dry Climate, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stuart Roy
Claire Halpin
7School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joanne Russell
1Cell and Molecular Sciences, The James Hutton Institute, Errol Road Invergowrie, Dundee, DD2 5DA, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caitlin Byrt
2ARC Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
8Research School of Biology, 46 Sullivans Creek Road, The Australian National University, Acton ACT 2601, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matt Gilliham
2ARC Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Matt Gilliham
  • For correspondence: robbie.waugh@hutton.ac.uk
David E. Salt
5Future Food Beacon of Excellence and the School of Biosciences, University of Nottingham, Nottingham NG7 2RD, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: robbie.waugh@hutton.ac.uk
Robbie Waugh
1Cell and Molecular Sciences, The James Hutton Institute, Errol Road Invergowrie, Dundee, DD2 5DA, Scotland, UK
3School of Agriculture and Wine & Waite Research Institute, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia
7School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: robbie.waugh@hutton.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

We quantified grain sodium (Na+) content across a barley GWAS panel grown under optimal conditions. We identified a strong association with a region containing two low and one high Na+ accumulating haplotypes of a Class 1 HIGH-AFFINITY POTASSIUM TRANSPORTER (HKT1;5) known to be involved in regulating plant Na+ homeostasis. The haplotypes exhibited an average 1.8-fold difference in grain Na+ content. We show that an L189P substitution disrupts Na+ transport in the high Na+ lines, disturbs the plasma membrane localisation typical of HKT1;5 and induces a conformational change in the protein predicted to compromise function. Under NaCl stress, lines containing P189 accumulate high levels of Na+, but show no significant difference in biomass. P189 increases in frequency from wild-species to elite cultivars leading us to speculate that the compromised haplotype is undergoing directional selection possibly due to the value of Na+ as a functional nutrient in non-saline environments.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 23, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A Grain of Salt
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A Grain of Salt
Kelly Houston, Jiaen Qiu, Stefanie Wege, Maria Hrmova, Helena Oakey, Yue Qu, Pauline Smith, Apriadi Situmorang, Malcolm Macaulay, Paulina Flis, Micha Bayer, Stuart Roy, Claire Halpin, Joanne Russell, Caitlin Byrt, Matt Gilliham, David E. Salt, Robbie Waugh
bioRxiv 2020.01.23.916742; doi: https://doi.org/10.1101/2020.01.23.916742
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A Grain of Salt
Kelly Houston, Jiaen Qiu, Stefanie Wege, Maria Hrmova, Helena Oakey, Yue Qu, Pauline Smith, Apriadi Situmorang, Malcolm Macaulay, Paulina Flis, Micha Bayer, Stuart Roy, Claire Halpin, Joanne Russell, Caitlin Byrt, Matt Gilliham, David E. Salt, Robbie Waugh
bioRxiv 2020.01.23.916742; doi: https://doi.org/10.1101/2020.01.23.916742

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4116)
  • Biochemistry (8820)
  • Bioengineering (6522)
  • Bioinformatics (23469)
  • Biophysics (11798)
  • Cancer Biology (9216)
  • Cell Biology (13327)
  • Clinical Trials (138)
  • Developmental Biology (7440)
  • Ecology (11417)
  • Epidemiology (2066)
  • Evolutionary Biology (15160)
  • Genetics (10441)
  • Genomics (14050)
  • Immunology (9176)
  • Microbiology (22168)
  • Molecular Biology (8817)
  • Neuroscience (47589)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3731)
  • Plant Biology (8083)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1254)