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HySyn: A genetically-encoded synthetic synapse to rewire neural circuits in vivo

View ORCID ProfileJosh D. Hawk, Daniel A. Colón-Ramos
doi: https://doi.org/10.1101/2020.01.27.922203
Josh D. Hawk
Grass Laboratory, Marine Biological Laboratory, Woods Hole, MA 02543, USADepartment of Neuroscience and Department of Cell Biology, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, P.O. Box 9812, New Haven, CT, 06536-0812, USA
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  • For correspondence: josh.hawk@yale.edu daniel.colon-ramos@yale.edu
Daniel A. Colón-Ramos
Department of Neuroscience and Department of Cell Biology, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, P.O. Box 9812, New Haven, CT, 06536-0812, USAInstituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico, 201 Blvd del Valle, San Juan, Puerto Rico
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  • For correspondence: josh.hawk@yale.edu daniel.colon-ramos@yale.edu
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Abstract

Here we introduce HySyn, a system designed to rewire neural connectivity in vivo by reconstituting a functional heterologous synapse. We demonstrate that genetically targeted expression of the two HySyn components, a Hydra-derived neuropeptide and its receptor, creates de novo neuromodulatory transmission in a mammalian neuronal tissue culture model and rewires a behavioral circuit in vivo in the nematode Caenorhabditis elegans. HySyn can interface with existing optogenetic, chemogenetic and pharmacological approaches to functionally probe synaptic transmission, dissect neuropeptide signaling, or modulate specific neural circuits.

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Posted January 28, 2020.
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HySyn: A genetically-encoded synthetic synapse to rewire neural circuits in vivo
Josh D. Hawk, Daniel A. Colón-Ramos
bioRxiv 2020.01.27.922203; doi: https://doi.org/10.1101/2020.01.27.922203
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HySyn: A genetically-encoded synthetic synapse to rewire neural circuits in vivo
Josh D. Hawk, Daniel A. Colón-Ramos
bioRxiv 2020.01.27.922203; doi: https://doi.org/10.1101/2020.01.27.922203

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