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Sonogenetics for noninvasive and cellular-level neuromodulation in rodent brain

Yaoheng Yang, Christopher Pham Pacia, Dezhuang Ye, Lifei Zhu, Hongchae Baek, Yimei Yue, Jinyun Yuan, Mark J. Miller, Jianmin Cui, Joseph P. Culver, Michael R. Bruchas, Hong Chen
doi: https://doi.org/10.1101/2020.01.28.919910
Yaoheng Yang
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Christopher Pham Pacia
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Dezhuang Ye
2Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Lifei Zhu
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Hongchae Baek
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Yimei Yue
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Jinyun Yuan
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Mark J. Miller
3Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA
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Jianmin Cui
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
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Joseph P. Culver
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
4Mallinckrodt Institute of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA
5Department of Physics, Washington University in St. Louis, Saint Louis, MO 63110, USA
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Michael R. Bruchas
6Department of Anesthesiology and Pain Medicine. Center for Neurobiology of Addiction, Pain, and Emotion. University of Washington, Seattle, WA 98195, USA
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Hong Chen
1Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA
7Department of Radiation Oncology, Washington University School of Medicine, Saint Louis, MO 63108, USA
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  • For correspondence: hongchen@wustl.edu
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Abstract

Sonogenetics, which uses ultrasound to noninvasively control cells genetically modified with ultrasound-sensitive ion channels, can be a powerful tool for investigating intact brain circuits. Here we show that TRPV1 is an ultrasound-sensitive ion channel that can modify the activity of TRPV1-expressing cells in vitro when exposing to focused ultrasound. We also show that focused ultrasound exposure at the mouse brain in vivo can selectively activate neurons that are genetically modified to express TRPV1. We demonstrate that precise manipulation of neural activity via TRPV1-based sonogenetics can be achieved by spatiotemporal control of ultrasound-induced heating. The focused ultrasound exposure is safe based on our inspection of neuronal integrity, apoptosis, and inflammation markers. This sonogenetic tool enables noninvasive, cell-type specific, spatiotemporally controlled modulation of mammalian brain activity.

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Posted January 28, 2020.
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Sonogenetics for noninvasive and cellular-level neuromodulation in rodent brain
Yaoheng Yang, Christopher Pham Pacia, Dezhuang Ye, Lifei Zhu, Hongchae Baek, Yimei Yue, Jinyun Yuan, Mark J. Miller, Jianmin Cui, Joseph P. Culver, Michael R. Bruchas, Hong Chen
bioRxiv 2020.01.28.919910; doi: https://doi.org/10.1101/2020.01.28.919910
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Sonogenetics for noninvasive and cellular-level neuromodulation in rodent brain
Yaoheng Yang, Christopher Pham Pacia, Dezhuang Ye, Lifei Zhu, Hongchae Baek, Yimei Yue, Jinyun Yuan, Mark J. Miller, Jianmin Cui, Joseph P. Culver, Michael R. Bruchas, Hong Chen
bioRxiv 2020.01.28.919910; doi: https://doi.org/10.1101/2020.01.28.919910

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