Abstract
Cellular metabolism encompasses the biochemical reactions and transportation of various metabolites in cells and their surroundings, which are integrated at all levels of cellular functions. We developed a method to systematically simulate cellular metabolism using single-cell RNA-seq (scRNA-seq) data through constraint-based context specific metabolic modeling. We simulated the NAD+ biosynthesis activity in 7 different mouse tissues, and the simulated NAD+ biosynthesis flux levels showed significant linear correlation with experimental measurements in previous research. We also show that the simulated NAD+ biosynthesis fluxes are reproducible using two additional scRNA-seq datasets.
Footnotes
Add legend to Figure 4.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.








