Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

G-baToN: a versatile cell-cell interaction reporter system

View ORCID ProfileRui Tang, Christopher W. Murray, Ian Linde, Nicholas J. Kramer, Min K. Tsai, Leo Chen, Hongchen Cai, Aaron D. Gitler, Edgar Engleman, Monte M. Winslow
doi: https://doi.org/10.1101/2020.02.01.929877
Rui Tang
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rui Tang
  • For correspondence: tangrui@stanford.edu mwinslow@stanford.edu
Christopher W. Murray
Cancer Biology Program, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ian Linde
Immunology Program, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicholas J. Kramer
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USANeuroscience Program, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Min K. Tsai
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leo Chen
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hongchen Cai
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aaron D. Gitler
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USANeuroscience Program, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edgar Engleman
Cancer Biology Program, Stanford University School of Medicine, Stanford, CA, USAImmunology Program, Stanford University School of Medicine, Stanford, CA, USADepartment of Pathology, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Monte M. Winslow
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USACancer Biology Program, Stanford University School of Medicine, Stanford, CA, USADepartment of Pathology, Stanford University School of Medicine, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: tangrui@stanford.edu mwinslow@stanford.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Cell-cell interactions influence all aspects of development, homeostasis, and disease. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon fluorescent protein transfer driven by nanobody-mediated recognition to enable sensitive and specific labeling of cells after cell-cell interactions. A suite of orthogonal baToN tools enables reciprocal cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher order cell-cell interactions across a wide range of cell types.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted February 02, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
G-baToN: a versatile cell-cell interaction reporter system
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
G-baToN: a versatile cell-cell interaction reporter system
Rui Tang, Christopher W. Murray, Ian Linde, Nicholas J. Kramer, Min K. Tsai, Leo Chen, Hongchen Cai, Aaron D. Gitler, Edgar Engleman, Monte M. Winslow
bioRxiv 2020.02.01.929877; doi: https://doi.org/10.1101/2020.02.01.929877
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
G-baToN: a versatile cell-cell interaction reporter system
Rui Tang, Christopher W. Murray, Ian Linde, Nicholas J. Kramer, Min K. Tsai, Leo Chen, Hongchen Cai, Aaron D. Gitler, Edgar Engleman, Monte M. Winslow
bioRxiv 2020.02.01.929877; doi: https://doi.org/10.1101/2020.02.01.929877

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioengineering
Subject Areas
All Articles
  • Animal Behavior and Cognition (1647)
  • Biochemistry (2738)
  • Bioengineering (1907)
  • Bioinformatics (10243)
  • Biophysics (4183)
  • Cancer Biology (3217)
  • Cell Biology (4538)
  • Clinical Trials (135)
  • Developmental Biology (2840)
  • Ecology (4460)
  • Epidemiology (2041)
  • Evolutionary Biology (7231)
  • Genetics (5475)
  • Genomics (6813)
  • Immunology (2388)
  • Microbiology (7480)
  • Molecular Biology (2992)
  • Neuroscience (18584)
  • Paleontology (136)
  • Pathology (472)
  • Pharmacology and Toxicology (780)
  • Physiology (1149)
  • Plant Biology (2706)
  • Scientific Communication and Education (680)
  • Synthetic Biology (888)
  • Systems Biology (2846)
  • Zoology (468)