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The cation diffusion facilitator protein MamM's cytoplasmic domain exhibits metal-type dependent binding modes and discriminates against Mn2+

Shiran Barber-Zucker, Jenny Hall, Afonso Froes, Sofiya Kolusheva, Fraser MacMillan, Raz Zarivach
doi: https://doi.org/10.1101/2020.02.02.930644
Shiran Barber-Zucker
Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;
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  • For correspondence: shiranbarber@gmail.com
Jenny Hall
University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
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  • For correspondence: jenny.hall@uea.ac.uk
Afonso Froes
University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
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Sofiya Kolusheva
Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;
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  • For correspondence: kolushev@gmail.com
Fraser MacMillan
University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
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  • For correspondence: fraser.macmillan@uea.ac.uk
Raz Zarivach
Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;
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  • For correspondence: zarivach@bgu.ac.il
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Abstract

Cation diffusion facilitator (CDF) proteins are a conserved family of divalent transition metal cation transporters. CDF proteins are usually composed of two domains: the transmembrane domain (TMD), in which the metal cations are transported through, and a regulatory cytoplasmic C-terminal domain (CTD). Each CDF protein transports either one specific metal, or multiple metals, from the cytoplasm. Here, the model CDF protein MamM, from magnetotactic bacteria, was used to probe the role of the CTD in metal selectivity. Using a combination of biophysical and structural approaches, the binding of different metals to MamM CTD was characterized. Results reveal that different metals bind distinctively to MamM CTD in terms of; their binding sites, thermodynamics and binding-dependent conformation, both in crystal form and in solution. Furthermore, the results indicate that the CTD discriminates against Mn2+ and provides the first direct evidence that CDF CTD's play a role in metal selectivity.

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Posted February 02, 2020.
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The cation diffusion facilitator protein MamM's cytoplasmic domain exhibits metal-type dependent binding modes and discriminates against Mn2+
Shiran Barber-Zucker, Jenny Hall, Afonso Froes, Sofiya Kolusheva, Fraser MacMillan, Raz Zarivach
bioRxiv 2020.02.02.930644; doi: https://doi.org/10.1101/2020.02.02.930644
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The cation diffusion facilitator protein MamM's cytoplasmic domain exhibits metal-type dependent binding modes and discriminates against Mn2+
Shiran Barber-Zucker, Jenny Hall, Afonso Froes, Sofiya Kolusheva, Fraser MacMillan, Raz Zarivach
bioRxiv 2020.02.02.930644; doi: https://doi.org/10.1101/2020.02.02.930644

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