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Impact of immediate cryopreservation on the establishment of patient derived xenografts from head and neck cancer patients

Lindsey Abel, View ORCID ProfileArda Durmaz, Rong Hu, Colin Longhurst, Andrew M. Baschnagel, Deric Wheeler, View ORCID ProfileJacob G. Scott, View ORCID ProfileRandall J. Kimple
doi: https://doi.org/10.1101/2020.02.03.930891
Lindsey Abel
1Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
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Arda Durmaz
5Departments of Translational Hematology and Oncology Research and Radiation Oncology, Cleveland Clinic, Cleveland, OH, USA
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Rong Hu
2Department of Pathology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
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Colin Longhurst
3Department of Biostatistics and Medical Informatics, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
4UW Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
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Andrew M. Baschnagel
1Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
4UW Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
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Deric Wheeler
1Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
4UW Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
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Jacob G. Scott
5Departments of Translational Hematology and Oncology Research and Radiation Oncology, Cleveland Clinic, Cleveland, OH, USA
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Randall J. Kimple
1Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
4UW Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
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  • For correspondence: rkimple@humonc.wisc.edu
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Abstract

Background Patient-derived xenografts established from human cancers are important tools for investigating novel anti-cancer therapies. Establishing PDXs requires a significant investment and many PDXs may be used infrequently due to their similarity to existing models, their growth rate, or the lack of relevant mutations. We performed this study to determine whether we could efficiently establish PDXs after cryopreservation to allow molecular profiling to be completed prior to implanting the human cancer.

Methods Fresh tumor was split with half used to establish a PDX immediately and half cryopreserved for later implantation. Resulting tumors were assessed histologically and tumors established from fresh or cryopreserved tissues compared as to the growth rate, extent of tumor necrosis, mitotic activity, keratinization, and grade. All PDXs were subjected to short tandem repeat testing to confirm identity and assess similarity between methods.

Results Tumor growth was seen in 70% of implanted cases. No growth in either condition was seen in 30% of tumors. One developed a SCC from the immediate implant but a lymphoproliferative mass without SCC from the cryopreserved specimen. No difference in growth rate was seen. No difference between histologic parameters was seen between the two approaches.

Conclusions Fresh human cancer tissue can be immediately cryopreserved and later thawed and implanted to establish PDXs. This resource saving approach allows for tumor profiling prior to implantation into animals thus maximizing the probability that the tumor will be utilized for future research.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 18, 2021.
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Impact of immediate cryopreservation on the establishment of patient derived xenografts from head and neck cancer patients
Lindsey Abel, Arda Durmaz, Rong Hu, Colin Longhurst, Andrew M. Baschnagel, Deric Wheeler, Jacob G. Scott, Randall J. Kimple
bioRxiv 2020.02.03.930891; doi: https://doi.org/10.1101/2020.02.03.930891
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Impact of immediate cryopreservation on the establishment of patient derived xenografts from head and neck cancer patients
Lindsey Abel, Arda Durmaz, Rong Hu, Colin Longhurst, Andrew M. Baschnagel, Deric Wheeler, Jacob G. Scott, Randall J. Kimple
bioRxiv 2020.02.03.930891; doi: https://doi.org/10.1101/2020.02.03.930891

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