New Results
Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936
View ORCID ProfileJamie Toombs, Lindsay Panther, Loren Ornelas, Chunyan Liu, Emilda Gomez, Raquel Martín-Ibáñez, Simon R. Cox, Stuart J. Ritchie, Sarah E. Harris, Adele Taylor, Paul Redmond, View ORCID ProfileTom C. Russ, Lee Murphy, James D. Cooper, Karen Burr, Bhuvaneish T. Selvaraj, Cathy Browne, Clive N. Svendsen, Sally A. Cowley, Ian J. Deary, Siddharthan Chandran, View ORCID ProfileTara Spires-Jones, Dhruv Sareen
doi: https://doi.org/10.1101/2020.02.05.935213
Jamie Toombs
aCentre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK
Lindsay Panther
biPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
cCedars-Sinai Biomanufacturing Center, West Hollywood, CA, 90069, USA
Loren Ornelas
biPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
cCedars-Sinai Biomanufacturing Center, West Hollywood, CA, 90069, USA
Chunyan Liu
biPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
cCedars-Sinai Biomanufacturing Center, West Hollywood, CA, 90069, USA
Emilda Gomez
biPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
cCedars-Sinai Biomanufacturing Center, West Hollywood, CA, 90069, USA
Raquel Martín-Ibáñez
cCedars-Sinai Biomanufacturing Center, West Hollywood, CA, 90069, USA
Simon R. Cox
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Stuart J. Ritchie
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Sarah E. Harris
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Adele Taylor
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Paul Redmond
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Tom C. Russ
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Lee Murphy
eEdinburgh Clinical Research Facility, University of Edinburgh, Edinburgh, UK
James D. Cooper
fDementia Research Institute at the University of Edinburgh, UK
kCentre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
Karen Burr
fDementia Research Institute at the University of Edinburgh, UK
kCentre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
Bhuvaneish T. Selvaraj
fDementia Research Institute at the University of Edinburgh, UK
kCentre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
Cathy Browne
iJames Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
Clive N. Svendsen
gBoard of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
hDepartment of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
Sally A. Cowley
iJames Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
jOxford Parkinson’s Disease Centre, Oxford, UK.
Ian J. Deary
dLothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
Siddharthan Chandran
fDementia Research Institute at the University of Edinburgh, UK
kCentre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
Tara Spires-Jones
aCentre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK
Dhruv Sareen
biPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
cCedars-Sinai Biomanufacturing Center, West Hollywood, CA, 90069, USA
gBoard of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
hDepartment of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
Abstract
Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Posted February 05, 2020.
Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936
Jamie Toombs, Lindsay Panther, Loren Ornelas, Chunyan Liu, Emilda Gomez, Raquel Martín-Ibáñez, Simon R. Cox, Stuart J. Ritchie, Sarah E. Harris, Adele Taylor, Paul Redmond, Tom C. Russ, Lee Murphy, James D. Cooper, Karen Burr, Bhuvaneish T. Selvaraj, Cathy Browne, Clive N. Svendsen, Sally A. Cowley, Ian J. Deary, Siddharthan Chandran, Tara Spires-Jones, Dhruv Sareen
bioRxiv 2020.02.05.935213; doi: https://doi.org/10.1101/2020.02.05.935213
Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936
Jamie Toombs, Lindsay Panther, Loren Ornelas, Chunyan Liu, Emilda Gomez, Raquel Martín-Ibáñez, Simon R. Cox, Stuart J. Ritchie, Sarah E. Harris, Adele Taylor, Paul Redmond, Tom C. Russ, Lee Murphy, James D. Cooper, Karen Burr, Bhuvaneish T. Selvaraj, Cathy Browne, Clive N. Svendsen, Sally A. Cowley, Ian J. Deary, Siddharthan Chandran, Tara Spires-Jones, Dhruv Sareen
bioRxiv 2020.02.05.935213; doi: https://doi.org/10.1101/2020.02.05.935213
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