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Engineering isoprenoid quinone production in yeast

View ORCID ProfileDivjot Kaur, View ORCID ProfileChristophe Corre, View ORCID ProfileFabrizio Alberti
doi: https://doi.org/10.1101/2020.02.06.932020
Divjot Kaur
†School of Life Sciences and Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
‡Department of Zoology, University of Oxford, Zoology Research and Administration Building, 11a Mansfield Road, Oxford OX1 3PS, UK
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Christophe Corre
†School of Life Sciences and Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
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Fabrizio Alberti
†School of Life Sciences and Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
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  • For correspondence: f.alberti@warwick.ac.uk
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ABSTRACT

Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also produced by all domains of life. Here, we report the engineering of a baker’s yeast strain, Saccharomyces cerevisiae EPYFA3, for the production of isoprenoid quinones. Our yeast strain was developed through overexpression of the shikimate pathway in a well-established recipient strain (S. cerevisiae EPY300) where the mevalonate pathway is overexpressed. As a proof of concept, our new host strain was used to overproduce the endogenous isoprenoid quinone coenzyme Q6, resulting in a final four-fold production increase. EPYFA3 represents a valuable platform for the heterologous production of high value isoprenoid quinones. EPYFA3 will also facilitate the elucidation of isoprenoid quinone biosynthetic pathways.

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Posted February 06, 2020.
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Engineering isoprenoid quinone production in yeast
Divjot Kaur, Christophe Corre, Fabrizio Alberti
bioRxiv 2020.02.06.932020; doi: https://doi.org/10.1101/2020.02.06.932020
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Engineering isoprenoid quinone production in yeast
Divjot Kaur, Christophe Corre, Fabrizio Alberti
bioRxiv 2020.02.06.932020; doi: https://doi.org/10.1101/2020.02.06.932020

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