Abstract
Purpose When developing new types of tools for single-cell analyses, there is often a lack of datasets on which to quantitatively assess the performance.
Results We developed dyngen, a multi-modality simulator of single cells. In dyngen, the biomolecular state of an in silico changes over time according to a predefined gene regulatory network. We used dyngen to benchmark three emerging ways of analysing single-cell data: RNA velocity, cell-specific network inference and trajectory alignment methods.
Conclusion dyngen lays the foundations for benchmarking a wide variety of computational single-cell tools and can be used to help kick-start the development of future types of analyses.
Footnotes
Added notification that the results of Figure 4A are currently being revised.