New Results
The Pathogenicity of 2019 Novel Coronavirus in hACE2 Transgenic Mice
Linlin Bao, Wei Deng, Baoying Huang, Hong Gao, Lili Ren, Qiang Wei, Pin Yu, Yanfeng Xu, Jiangning Liu, Feifei Qi, Yajin Qu, Wenling Wang, Fengdi Li, Qi Lv, Jing Xue, Shuran Gong, Mingya Liu, Guanpeng Wang, Shunyi Wang, Linna Zhao, Peipei Liu, Li Zhao, Fei Ye, Huijuan Wang, Weimin Zhou, Na Zhu, Wei Zhen, Xiaojuan Zhang, Zhiqi Song, Li Guo, Lan Chen, Conghui Wang, Ying Wang, Xinming Wang, Yan Xiao, Qiangming Sun, Hongqi Liu, Fanli Zhu, Chunxia Ma, Lingmei Yan, Mengli Yang, Jun Han, Wenbo Xu, Wenjie Tan, Xiaozhong Peng, Qi Jin, Guizhen Wu, Chuan Qin
doi: https://doi.org/10.1101/2020.02.07.939389
Linlin Bao
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Wei Deng
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Baoying Huang
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Hong Gao
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Lili Ren
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Qiang Wei
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Pin Yu
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Yanfeng Xu
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Jiangning Liu
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Feifei Qi
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Yajin Qu
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Wenling Wang
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Fengdi Li
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Qi Lv
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Jing Xue
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Shuran Gong
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Mingya Liu
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Guanpeng Wang
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Shunyi Wang
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Linna Zhao
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Peipei Liu
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Li Zhao
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Fei Ye
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Huijuan Wang
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Weimin Zhou
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Na Zhu
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Wei Zhen
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Xiaojuan Zhang
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Zhiqi Song
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Li Guo
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Lan Chen
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Conghui Wang
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Ying Wang
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Xinming Wang
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Yan Xiao
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Qiangming Sun
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Hongqi Liu
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Fanli Zhu
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Chunxia Ma
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Lingmei Yan
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Mengli Yang
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Jun Han
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Wenbo Xu
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Wenjie Tan
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Xiaozhong Peng
4Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China
Qi Jin
3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China
Guizhen Wu
2MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
Chuan Qin
1Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China
Abstract
2019-nCoV caused pneumonia cases in China has become a public health emergency of international concern (PHEIC). The first priority for prevention and treatment of the disease is to find the pathogenicity of 2019-nCoV in vivo. Weight loss and virus replication were detected in infected-hACE2 mice. The typical histopathology was interstitial pneumonia with significant inflammatory cells infiltration around the bronchioles and blood vessels, and viral antigens were observed in bronchial epithelial cells and alveolar epithelial cells. The phenomenon was not found in wild type mice infected with 2019-nCoV and the mock-infected hACE2 mice. The pathogenicity of 2019-nCoV in hACE2 mice was clarified and the Koch’s postulates was fulfilled as well, and the model may facilitate the development of therapeutics and vaccines against 2019-nCoV.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted February 11, 2020.
The Pathogenicity of 2019 Novel Coronavirus in hACE2 Transgenic Mice
Linlin Bao, Wei Deng, Baoying Huang, Hong Gao, Lili Ren, Qiang Wei, Pin Yu, Yanfeng Xu, Jiangning Liu, Feifei Qi, Yajin Qu, Wenling Wang, Fengdi Li, Qi Lv, Jing Xue, Shuran Gong, Mingya Liu, Guanpeng Wang, Shunyi Wang, Linna Zhao, Peipei Liu, Li Zhao, Fei Ye, Huijuan Wang, Weimin Zhou, Na Zhu, Wei Zhen, Xiaojuan Zhang, Zhiqi Song, Li Guo, Lan Chen, Conghui Wang, Ying Wang, Xinming Wang, Yan Xiao, Qiangming Sun, Hongqi Liu, Fanli Zhu, Chunxia Ma, Lingmei Yan, Mengli Yang, Jun Han, Wenbo Xu, Wenjie Tan, Xiaozhong Peng, Qi Jin, Guizhen Wu, Chuan Qin
bioRxiv 2020.02.07.939389; doi: https://doi.org/10.1101/2020.02.07.939389
The Pathogenicity of 2019 Novel Coronavirus in hACE2 Transgenic Mice
Linlin Bao, Wei Deng, Baoying Huang, Hong Gao, Lili Ren, Qiang Wei, Pin Yu, Yanfeng Xu, Jiangning Liu, Feifei Qi, Yajin Qu, Wenling Wang, Fengdi Li, Qi Lv, Jing Xue, Shuran Gong, Mingya Liu, Guanpeng Wang, Shunyi Wang, Linna Zhao, Peipei Liu, Li Zhao, Fei Ye, Huijuan Wang, Weimin Zhou, Na Zhu, Wei Zhen, Xiaojuan Zhang, Zhiqi Song, Li Guo, Lan Chen, Conghui Wang, Ying Wang, Xinming Wang, Yan Xiao, Qiangming Sun, Hongqi Liu, Fanli Zhu, Chunxia Ma, Lingmei Yan, Mengli Yang, Jun Han, Wenbo Xu, Wenjie Tan, Xiaozhong Peng, Qi Jin, Guizhen Wu, Chuan Qin
bioRxiv 2020.02.07.939389; doi: https://doi.org/10.1101/2020.02.07.939389
Subject Area
Subject Areas
- Biochemistry (13669)
- Bioengineering (10399)
- Bioinformatics (33085)
- Biophysics (17058)
- Cancer Biology (14123)
- Cell Biology (20045)
- Clinical Trials (138)
- Developmental Biology (10840)
- Ecology (15971)
- Epidemiology (2067)
- Evolutionary Biology (20303)
- Genetics (13375)
- Genomics (18602)
- Immunology (13708)
- Microbiology (32082)
- Molecular Biology (13357)
- Neuroscience (69908)
- Paleontology (523)
- Pathology (2178)
- Pharmacology and Toxicology (3729)
- Physiology (5847)
- Plant Biology (11993)
- Synthetic Biology (3359)
- Systems Biology (8146)
- Zoology (1840)