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A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Philip M. Borden, Peng Zhang, View ORCID ProfileAmol V. Shivange, View ORCID ProfileJonathan S. Marvin, View ORCID ProfileJoseph Cichon, Chuntao Dan, View ORCID ProfileKaspar Podgorski, Antonio Figueiredo, View ORCID ProfileOndrej Novak, Masashi Tanimoto, View ORCID ProfileEiji Shigetomi, Mark A. Lobas, Hyuntae Kim, Paula K. Zhu, Yajun Zhang, W. Sharon Zheng, View ORCID ProfileChengCheng Fan, Guangfu Wang, Bowen Xiang, Li Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G. Yee, View ORCID ProfileAbhi Aggarwal, Christopher P. Ford, View ORCID ProfileDouglas C. Rees, View ORCID ProfileDirk Dietrich, View ORCID ProfileBaljit S. Khakh, View ORCID ProfileJeremy S. Dittman, View ORCID ProfileWen-Biao Gan, View ORCID ProfileMinoru Koyama, View ORCID ProfileVivek Jayaraman, View ORCID ProfileJoseph F. Cheer, View ORCID ProfileHenry A. Lester, View ORCID ProfileJ. Julius Zhu, View ORCID ProfileLoren L. Looger
doi: https://doi.org/10.1101/2020.02.07.939504
Philip M. Borden
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
26LifeEDIT, Research Triangle Park, North Carolina, USA
Peng Zhang
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
Amol V. Shivange
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
3Division of Biology and Biological Engineering, California Institute of Technology, Pasadena California, USA
Jonathan S. Marvin
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Joseph Cichon
4Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York, USA
5Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania, USA
Chuntao Dan
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Kaspar Podgorski
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Antonio Figueiredo
6Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland, USA
Ondrej Novak
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
27Second Faculty of Medicine, Charles University, Prague, Czech Republic
Masashi Tanimoto
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Eiji Shigetomi
7Department of Physiology and Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, California, USA
8Department of Neuropharmacology, University of Yamanashi, Yamanashi, Japan
Mark A. Lobas
7Department of Physiology and Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, California, USA
28Koniku Inc., Berkeley, California, USA
Hyuntae Kim
9Department of Neurosurgery, University Clinic Bonn, Bonn, Germany
Paula K. Zhu
10Math, Engineering & Science Academy Class of 2020, Albemarle High School, Charlottesville, Virginia, USA
11Summer Secondary School Neurobiology Class of 2019, Harvard University, Cambridge, Massachusetts, USA
Yajun Zhang
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
W. Sharon Zheng
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
12Biomedical Engineering Class of 2021, University of Virginia School of Engineering and Applied Sciences, Charlottesville, Virginia, USA
ChengCheng Fan
13Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA
Guangfu Wang
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
Bowen Xiang
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
14Biomedical Engineering Class of 2020, University of Virginia School of Engineering and Applied Sciences, Charlottesville, Virginia, USA
Li Gan
15Helen and Robert Appel Alzheimer’s Disease Research Institute, Weill Cornell Medicine College, New York, New York, USA
Guang-Xian Zhang
16Department of Neurology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
Kaiming Guo
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
17School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China
Li Lin
17School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China
Yuan Cai
18Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA
Andrew G. Yee
18Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA
Abhi Aggarwal
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
19Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Christopher P. Ford
18Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA
Douglas C. Rees
13Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA
20Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA
Dirk Dietrich
9Department of Neurosurgery, University Clinic Bonn, Bonn, Germany
Baljit S. Khakh
7Department of Physiology and Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, California, USA
Jeremy S. Dittman
21Department of Biochemistry, Weill Cornell Medical College, New York, New York, USA
Wen-Biao Gan
4Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York, USA
Minoru Koyama
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Vivek Jayaraman
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Joseph F. Cheer
6Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland, USA
22Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland, USA
23Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland, USA
Henry A. Lester
3Division of Biology and Biological Engineering, California Institute of Technology, Pasadena California, USA
J. Julius Zhu
2Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA
24School of Medicine, Ningbo University, Ningbo, China
25Donders Institute for Brain, Cognition and Behavior, Radboud University, Nijmegen, Netherlands
Loren L. Looger
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Abstract
Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all animals.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted February 08, 2020.
A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Philip M. Borden, Peng Zhang, Amol V. Shivange, Jonathan S. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novak, Masashi Tanimoto, Eiji Shigetomi, Mark A. Lobas, Hyuntae Kim, Paula K. Zhu, Yajun Zhang, W. Sharon Zheng, ChengCheng Fan, Guangfu Wang, Bowen Xiang, Li Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G. Yee, Abhi Aggarwal, Christopher P. Ford, Douglas C. Rees, Dirk Dietrich, Baljit S. Khakh, Jeremy S. Dittman, Wen-Biao Gan, Minoru Koyama, Vivek Jayaraman, Joseph F. Cheer, Henry A. Lester, J. Julius Zhu, Loren L. Looger
bioRxiv 2020.02.07.939504; doi: https://doi.org/10.1101/2020.02.07.939504
A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Philip M. Borden, Peng Zhang, Amol V. Shivange, Jonathan S. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novak, Masashi Tanimoto, Eiji Shigetomi, Mark A. Lobas, Hyuntae Kim, Paula K. Zhu, Yajun Zhang, W. Sharon Zheng, ChengCheng Fan, Guangfu Wang, Bowen Xiang, Li Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G. Yee, Abhi Aggarwal, Christopher P. Ford, Douglas C. Rees, Dirk Dietrich, Baljit S. Khakh, Jeremy S. Dittman, Wen-Biao Gan, Minoru Koyama, Vivek Jayaraman, Joseph F. Cheer, Henry A. Lester, J. Julius Zhu, Loren L. Looger
bioRxiv 2020.02.07.939504; doi: https://doi.org/10.1101/2020.02.07.939504
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