Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

Takayuki Tamaki, Satoyo Oya, Makiko Naito, Yasuko Ozawa, Tomoyuki Furuya, Masato Saito, Mayuko Sato, Mayumi Wakazaki, Kiminori Toyooka, Hiroo Fukuda, Ykä Helariutta, Yuki Kondo
doi: https://doi.org/10.1101/2020.02.08.939595
Takayuki Tamaki
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Satoyo Oya
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Makiko Naito
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasuko Ozawa
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tomoyuki Furuya
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masato Saito
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mayuko Sato
2RIKEN Centre for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa, Yokohama 230-0045, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mayumi Wakazaki
2RIKEN Centre for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa, Yokohama 230-0045, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiminori Toyooka
2RIKEN Centre for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa, Yokohama 230-0045, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroo Fukuda
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ykä Helariutta
3Institute of Biotechnology/Department of Biological and Environmental Sciences, University of Helsinki, FIN-00014, Finland
4The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuki Kondo
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: p@bs.s.u-tokyo.ac.jp
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The phloem transports photosynthetic assimilates and signalling molecules. It mainly consists of sieve elements (SEs), which act as “highways” for transport, and companion cells (CCs), which serve as “gates” to load/unload cargos. Though SEs and CCs function together, it remains unknown what determines the ratio of SE/CC in the phloem. In this study, we develop a novel culture system for CC differentiation named VISUAL-CC, which reconstitutes the SE-CC complex formation. Comparative expression analysis in VISUAL-CC reveals that SE and CC differentiation tends to show negative correlation, while total phloem differentiation is unchanged. This varying SE/CC ratio is largely dependent on GSK3 kinase activity. Indeed, gsk3 hextuple mutants possess much more SEs and less CCs in planta. Conversely, gsk3 gain-of-function mutants induced by phloem-specific promoter partially increased the CC ratio. Taken together, GSK3 activity appears to function as a cell fate switch in the phloem, thereby balancing the SE/CC ratio.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted February 10, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
GSK3 activity is a cell fate switch that balances the ratio of vascular cell type
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
GSK3 activity is a cell fate switch that balances the ratio of vascular cell type
Takayuki Tamaki, Satoyo Oya, Makiko Naito, Yasuko Ozawa, Tomoyuki Furuya, Masato Saito, Mayuko Sato, Mayumi Wakazaki, Kiminori Toyooka, Hiroo Fukuda, Ykä Helariutta, Yuki Kondo
bioRxiv 2020.02.08.939595; doi: https://doi.org/10.1101/2020.02.08.939595
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
GSK3 activity is a cell fate switch that balances the ratio of vascular cell type
Takayuki Tamaki, Satoyo Oya, Makiko Naito, Yasuko Ozawa, Tomoyuki Furuya, Masato Saito, Mayuko Sato, Mayumi Wakazaki, Kiminori Toyooka, Hiroo Fukuda, Ykä Helariutta, Yuki Kondo
bioRxiv 2020.02.08.939595; doi: https://doi.org/10.1101/2020.02.08.939595

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3482)
  • Biochemistry (7329)
  • Bioengineering (5301)
  • Bioinformatics (20212)
  • Biophysics (9985)
  • Cancer Biology (7706)
  • Cell Biology (11273)
  • Clinical Trials (138)
  • Developmental Biology (6425)
  • Ecology (9923)
  • Epidemiology (2065)
  • Evolutionary Biology (13292)
  • Genetics (9353)
  • Genomics (12559)
  • Immunology (7681)
  • Microbiology (18964)
  • Molecular Biology (7421)
  • Neuroscience (40915)
  • Paleontology (298)
  • Pathology (1226)
  • Pharmacology and Toxicology (2130)
  • Physiology (3145)
  • Plant Biology (6842)
  • Scientific Communication and Education (1271)
  • Synthetic Biology (1893)
  • Systems Biology (5299)
  • Zoology (1086)