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Valorization of CO2 through lithoautotrophic production of sustainable chemicals in Cupriavidus necator

Shannon N. Nangle, Marika Ziesack, Sarabeth Buckley, Disha Trivedi, Daniel M. Loh, Daniel G. Nocera, Pamela A. Silver
doi: https://doi.org/10.1101/2020.02.08.940007
Shannon N. Nangle
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA
2Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA
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Marika Ziesack
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA
2Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA
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Sarabeth Buckley
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA
3Department of Earth and Environment, Boston University, Boston, MA, USA
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Disha Trivedi
4Faculty of Arts and Sciences, Harvard University, Boston, MA, USA
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Daniel M. Loh
5Department of Chemistry, Harvard Medical School, Boston, MA, USA
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Daniel G. Nocera
5Department of Chemistry, Harvard Medical School, Boston, MA, USA
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Pamela A. Silver
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA
2Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA
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  • For correspondence: pamela_silver@hms.harvard.edu
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Abstract

Coupling recent advancements in genetic engineering of diverse microbes and gas-driven fermentation provides a path towards sustainable commodity chemical production. Cupriavidus necator H16 is a suitable species for this task because it effectively utilizes H2 and CO2 and is genetically tractable. Here, we demonstrate the versatility of C. necator for chemical production by engineering it to produce three products from CO2 under lithotrophic conditions: sucrose, polyhydroxyalkanoates (PHAs), and lipochitooligosaccharides (LCOs). We engineered sucrose production in a co-culture system with heterotrophic growth 30 times that of WT C. necator. We engineered PHA production (20-60% DCW) and selectively altered product composition by combining different thioesterases and phaCs to produce copolymers directly from CO2. And, we engineered C. necator to convert CO2 into the LCO, a plant growth enhancer, with titers of ∼1.4 mg/L—equivalent to yields in its native source, Bradyrhizobium. We applied the LCOs to germinating seeds as well as corn plants and observed increases in a variety of growth parameters. Taken together, these results expand our understanding f how a gas-utilizing bacteria can promote sustainable production.

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Posted February 10, 2020.
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Valorization of CO2 through lithoautotrophic production of sustainable chemicals in Cupriavidus necator
Shannon N. Nangle, Marika Ziesack, Sarabeth Buckley, Disha Trivedi, Daniel M. Loh, Daniel G. Nocera, Pamela A. Silver
bioRxiv 2020.02.08.940007; doi: https://doi.org/10.1101/2020.02.08.940007
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Valorization of CO2 through lithoautotrophic production of sustainable chemicals in Cupriavidus necator
Shannon N. Nangle, Marika Ziesack, Sarabeth Buckley, Disha Trivedi, Daniel M. Loh, Daniel G. Nocera, Pamela A. Silver
bioRxiv 2020.02.08.940007; doi: https://doi.org/10.1101/2020.02.08.940007

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