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The Neural Basis for a Persistent Internal State in Drosophila Females

View ORCID ProfileDavid Deutsch, View ORCID ProfileDiego A. Pacheco, Lucas J. Encarnacion-Rivera, View ORCID ProfileTalmo Pereira, Ramie Fathy, View ORCID ProfileAdam Calhoun, Elise C. Ireland, Austin T. Burke, View ORCID ProfileSven Dorkenwald, View ORCID ProfileClaire McKellar, View ORCID ProfileThomas Macrina, Ran Lu, Kisuk Lee, Nico Kemnitz, Dodam Ih, Manuel Castro, Akhilesh Halageri, Chris Jordan, William Silversmith, Jingpeng Wu, H. Sebastian Seung, View ORCID ProfileMala Murthy
doi: https://doi.org/10.1101/2020.02.13.947952
David Deutsch
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Diego A. Pacheco
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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  • ORCID record for Diego A. Pacheco
Lucas J. Encarnacion-Rivera
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Talmo Pereira
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Ramie Fathy
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Adam Calhoun
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Elise C. Ireland
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Austin T. Burke
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Sven Dorkenwald
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
2Department of Computer Science, Princeton University, Princeton, NJ, USA
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Claire McKellar
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Thomas Macrina
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
2Department of Computer Science, Princeton University, Princeton, NJ, USA
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Ran Lu
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Kisuk Lee
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Nico Kemnitz
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Dodam Ih
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Manuel Castro
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Akhilesh Halageri
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Chris Jordan
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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William Silversmith
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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Jingpeng Wu
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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H. Sebastian Seung
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
2Department of Computer Science, Princeton University, Princeton, NJ, USA
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Mala Murthy
1Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
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  • ORCID record for Mala Murthy
  • For correspondence: mmurthy@princeton.edu
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Abstract

Sustained changes in mood or action require persistent changes in neural activity, but it has been difficult to identify and characterize the neural circuit mechanisms that underlie persistent activity and contribute to long-lasting changes in behavior. Here, we focus on changes in the behavioral state of Drosophila females that persist for minutes following optogenetic activation of a single class of central brain neurons termed pC1. We find that female pC1 neurons drive a variety of persistent behaviors in the presence of males, including increased receptivity, shoving, and chasing. By reconstructing cells in a volume electron microscopic image of the female brain, we classify 7 different pC1 cell types and, using cell type specific driver lines, determine that one of these, pC1-Alpha, is responsible for driving persistent female shoving and chasing. Using calcium imaging, we locate sites of minutes-long persistent neural activity in the brain, which include pC1 neurons themselves. Finally, we exhaustively reconstruct all synaptic partners of a single pC1-Alpha neuron, and find recurrent connectivity that could support the persistent neural activity. Our work thus links minutes-long persistent changes in behavior with persistent neural activity and recurrent circuit architecture in the female brain.

Footnotes

  • https://www.dropbox.com/sh/1n8zhumr5bhgbmz/AABAs0lh5MulBKVKJc16P0P8a?dl=0

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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The Neural Basis for a Persistent Internal State in Drosophila Females
David Deutsch, Diego A. Pacheco, Lucas J. Encarnacion-Rivera, Talmo Pereira, Ramie Fathy, Adam Calhoun, Elise C. Ireland, Austin T. Burke, Sven Dorkenwald, Claire McKellar, Thomas Macrina, Ran Lu, Kisuk Lee, Nico Kemnitz, Dodam Ih, Manuel Castro, Akhilesh Halageri, Chris Jordan, William Silversmith, Jingpeng Wu, H. Sebastian Seung, Mala Murthy
bioRxiv 2020.02.13.947952; doi: https://doi.org/10.1101/2020.02.13.947952
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The Neural Basis for a Persistent Internal State in Drosophila Females
David Deutsch, Diego A. Pacheco, Lucas J. Encarnacion-Rivera, Talmo Pereira, Ramie Fathy, Adam Calhoun, Elise C. Ireland, Austin T. Burke, Sven Dorkenwald, Claire McKellar, Thomas Macrina, Ran Lu, Kisuk Lee, Nico Kemnitz, Dodam Ih, Manuel Castro, Akhilesh Halageri, Chris Jordan, William Silversmith, Jingpeng Wu, H. Sebastian Seung, Mala Murthy
bioRxiv 2020.02.13.947952; doi: https://doi.org/10.1101/2020.02.13.947952

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