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LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

Acharya Balkrishna, Alpana Joshi, View ORCID ProfileSubrata K. Das, Laxmi Bisht, Sachin Sakat, Vinamra Sharma, Niti Sharma, Khemraj Joshi, Sudeep Verma, Vinay K Sharma, CS Joshi
doi: https://doi.org/10.1101/2020.02.15.950865
Acharya Balkrishna
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Alpana Joshi
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
2Department of Agriculture & AgriInformatics, School of Biological Engineering & Sciences Shobhit Deemed University, Meerut-250110, India
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Subrata K. Das
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
3School of Medical science and Technology, IIT Kharagpur-721302, India
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  • For correspondence: subratakdas09@gmail.com
Laxmi Bisht
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Sachin Sakat
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Vinamra Sharma
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
4Amity Institute of Indian System of Medicine, Amity University, Noida-201313, India
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Niti Sharma
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Khemraj Joshi
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Sudeep Verma
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Vinay K Sharma
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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CS Joshi
1Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar-249405, India
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Abstract

The incidence rate of diabetes mellitus is increasing worldwide. Herbal formulations have recently gained importance as an alternative therapeutic option in controlling diabetes without causing any side effects. In the present study, we have demonstrated maintenance of glycemic homeostasis and anti-adipogenic potential of a herbo-mineral formulation Divya MadhuKalp (DMK). Initially, we evaluated the presence of bioactive compounds in DMK using LC/MS-QToF analysis. In-vitro analysis of DMK in L6 (skeletal muscle) cells showed a significant increase in cellular glucose uptake. Similarly, a human equivalent dose of DMK significantly reduced blood glucose level in normoglycemic and oral glucose tolerance rat model. DMK extract also inhibited formation of advanced glycation end product and showed anti-α-glucosidase activity. Further analysis of DMK in 3T3 L1 pre-adipocytes demonstrated anti-adipogenic activity through reduction in intracellular lipid accumulation and triglyceride contents along with downregulation of major adipogenic transcriptional factors (PPAR-γ and C/EBPα) and, adipocytes marker genes (LPL, AP2 and adiponectin). In conclusion, DMK exhibited anti-diabetic and anti-adipogenic activities by synergistic effect of its bioactive compounds and can be considered as a potent herbo-mineral formulation for treating metabolic diseases.

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Posted March 19, 2020.
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LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation
Acharya Balkrishna, Alpana Joshi, Subrata K. Das, Laxmi Bisht, Sachin Sakat, Vinamra Sharma, Niti Sharma, Khemraj Joshi, Sudeep Verma, Vinay K Sharma, CS Joshi
bioRxiv 2020.02.15.950865; doi: https://doi.org/10.1101/2020.02.15.950865
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LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation
Acharya Balkrishna, Alpana Joshi, Subrata K. Das, Laxmi Bisht, Sachin Sakat, Vinamra Sharma, Niti Sharma, Khemraj Joshi, Sudeep Verma, Vinay K Sharma, CS Joshi
bioRxiv 2020.02.15.950865; doi: https://doi.org/10.1101/2020.02.15.950865

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