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Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube

Andrew C Symonds, View ORCID ProfileClare E Buckley, Charlotte A Williams, Jonathan DW Clarke
doi: https://doi.org/10.1101/2020.02.16.950543
Andrew C Symonds
2Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King’s College London, Guy’s Campus, London, SE1 1UL
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Clare E Buckley
2Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King’s College London, Guy’s Campus, London, SE1 1UL
3Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3BY
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  • ORCID record for Clare E Buckley
  • For correspondence: jon.clarke@kcl.ac.uk
Charlotte A Williams
2Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King’s College London, Guy’s Campus, London, SE1 1UL
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Jonathan DW Clarke
2Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King’s College London, Guy’s Campus, London, SE1 1UL
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  • For correspondence: jon.clarke@kcl.ac.uk
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Abstract

Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that NOK/Pals1/Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Nok and Rab11a mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose these roles for both Nok and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction remodelling role of Nok is not specific to dividing cells.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • rewrite of the text and additional analyses. addition of supplementary figure and movies

  • https://github.com/andyivanhoe/pard3-analysis

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 07, 2020.
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Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube
Andrew C Symonds, Clare E Buckley, Charlotte A Williams, Jonathan DW Clarke
bioRxiv 2020.02.16.950543; doi: https://doi.org/10.1101/2020.02.16.950543
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Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube
Andrew C Symonds, Clare E Buckley, Charlotte A Williams, Jonathan DW Clarke
bioRxiv 2020.02.16.950543; doi: https://doi.org/10.1101/2020.02.16.950543

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