Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development

Chunyun Sun, Long Chen, Ji Yang, Chunxia Luo, Yanjing Zhang, Jing Li, Jiahui Yang, Jie Zhang, Liangzhi Xie
doi: https://doi.org/10.1101/2020.02.16.951723
Chunyun Sun
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Long Chen
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ji Yang
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chunxia Luo
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yanjing Zhang
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jing Li
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiahui Yang
2Beijing Antibody Research Key Laboratory, Sino Biological Inc., Building 9, Jing Dong Bei Technology Park, No.18 Ke Chuang 10th St, BDA, Beijing, 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jie Zhang
2Beijing Antibody Research Key Laboratory, Sino Biological Inc., Building 9, Jing Dong Bei Technology Park, No.18 Ke Chuang 10th St, BDA, Beijing, 100176, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liangzhi Xie
1Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Ke Chuang 7th Street, Beijing 100176, China
2Beijing Antibody Research Key Laboratory, Sino Biological Inc., Building 9, Jing Dong Bei Technology Park, No.18 Ke Chuang 10th St, BDA, Beijing, 100176, China
3Cell Culture Engineering Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: liangzhi@yahoo.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

SARS-CoV-2 and SARS-CoV share a common human receptor ACE2. Protein-protein interaction structure modeling indicates that spike-RBD of the two viruses also has similar overall binding conformation and binding free energy to ACE2. In vitro assays using recombinant ACE2 proteins and ACE2 expressing cells confirmed the two coronaviruses’ similar binding affinities to ACE2. The above studies provide experimental supporting evidences and possible explanation for the high transmissibility observed in the SARS-CoV-2 outbreak. Potent ACE2-blocking SARS-CoV neutralizing antibodies showed limited cross-binding and neutralizing activities to SARS-CoV-2. ACE2-non-blocking SARS-CoV RBD antibodies, though with weaker neutralizing activities against SARS-CoV, showed positive cross-neutralizing activities to SARS-CoV-2 with an unknown mechanism. These findings suggest a trade-off between the efficacy and spectrum for therapeutic antibodies to different coronaviruses, and hence highlight the possibilities and challenges in developing broadly protecting antibodies and vaccines against SARS-CoV-2 and its future mutants.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted February 20, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development
Chunyun Sun, Long Chen, Ji Yang, Chunxia Luo, Yanjing Zhang, Jing Li, Jiahui Yang, Jie Zhang, Liangzhi Xie
bioRxiv 2020.02.16.951723; doi: https://doi.org/10.1101/2020.02.16.951723
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development
Chunyun Sun, Long Chen, Ji Yang, Chunxia Luo, Yanjing Zhang, Jing Li, Jiahui Yang, Jie Zhang, Liangzhi Xie
bioRxiv 2020.02.16.951723; doi: https://doi.org/10.1101/2020.02.16.951723

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4866)
  • Biochemistry (10821)
  • Bioengineering (8068)
  • Bioinformatics (27381)
  • Biophysics (14030)
  • Cancer Biology (11166)
  • Cell Biology (16106)
  • Clinical Trials (138)
  • Developmental Biology (8808)
  • Ecology (13332)
  • Epidemiology (2067)
  • Evolutionary Biology (17399)
  • Genetics (11705)
  • Genomics (15964)
  • Immunology (11061)
  • Microbiology (26169)
  • Molecular Biology (10680)
  • Neuroscience (56748)
  • Paleontology (422)
  • Pathology (1737)
  • Pharmacology and Toxicology (3012)
  • Physiology (4569)
  • Plant Biology (9670)
  • Scientific Communication and Education (1617)
  • Synthetic Biology (2699)
  • Systems Biology (6997)
  • Zoology (1515)