Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera

View ORCID ProfileUlrich Hohmann, View ORCID ProfilePriya Ramakrishna, View ORCID ProfileKai Wang, View ORCID ProfileLaura Lorenzo-Orts, View ORCID ProfileJoel Nicolet, View ORCID ProfileAgnes Henschen, View ORCID ProfileMarie Barberon, View ORCID ProfileMartin Bayer, View ORCID ProfileMichael Hothorn
doi: https://doi.org/10.1101/2020.02.18.954479
Ulrich Hohmann
1Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ulrich Hohmann
Priya Ramakrishna
1Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Priya Ramakrishna
Kai Wang
2Department of Cell Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kai Wang
Laura Lorenzo-Orts
1Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Laura Lorenzo-Orts
Joel Nicolet
1Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joel Nicolet
Agnes Henschen
2Department of Cell Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Agnes Henschen
Marie Barberon
1Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marie Barberon
Martin Bayer
2Department of Cell Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Martin Bayer
  • For correspondence: martin.bayer@tuebingen.mpg.de michael.hothorn@unige.ch
Michael Hothorn
1Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael Hothorn
  • For correspondence: martin.bayer@tuebingen.mpg.de michael.hothorn@unige.ch
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Receptor kinases with extracellular leucine-rich repeat domains (LRR-RKs) form the largest group of membrane signaling proteins in plants. LRR-RKs can sense small molecule, peptide or protein ligands, and may be activated by ligand-induced interaction with a shape complementary SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptor kinase. We have previously shown that SERKs can also form constitutive, ligand-independent complexes with the LRR ectodomains of BAK1-interacting receptor-like kinase 3 (BIR3) receptor pseudokinases, negative regulators of LRR-RK signaling. Here we report that receptor chimaera in which the extracellular LRR domain of BIR3 is fused to the cytoplasmic kinase domains of the SERK-dependent LRR-RKs BRASSINOSTEROID INSENSITIVE1, HAESA and ERECTA form tight complexes with endogenous SERK co-receptors in the absence of ligand stimulus. Expression of these chimaera under the control of the endogenous promoter of the respective LRR-RK leads to strong gain-of-function brassinosteroid, floral abscission and stomatal patterning phenotypes, respectively. Importantly, a BIR3-GSO1/SGN3 chimera can partially complement sgn3 Casparian strip formation phenotypes, suggesting that GSO1/SGN3 receptor activation is also mediated by SERK proteins. Collectively, our protein engineering approach may be used to elucidate the physiological functions of orphan LRR-RKs and to identify their receptor activation mechanism in single transgenic lines.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted February 19, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera
Ulrich Hohmann, Priya Ramakrishna, Kai Wang, Laura Lorenzo-Orts, Joel Nicolet, Agnes Henschen, Marie Barberon, Martin Bayer, Michael Hothorn
bioRxiv 2020.02.18.954479; doi: https://doi.org/10.1101/2020.02.18.954479
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera
Ulrich Hohmann, Priya Ramakrishna, Kai Wang, Laura Lorenzo-Orts, Joel Nicolet, Agnes Henschen, Marie Barberon, Martin Bayer, Michael Hothorn
bioRxiv 2020.02.18.954479; doi: https://doi.org/10.1101/2020.02.18.954479

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3484)
  • Biochemistry (7336)
  • Bioengineering (5308)
  • Bioinformatics (20225)
  • Biophysics (9991)
  • Cancer Biology (7717)
  • Cell Biology (11280)
  • Clinical Trials (138)
  • Developmental Biology (6426)
  • Ecology (9930)
  • Epidemiology (2065)
  • Evolutionary Biology (13298)
  • Genetics (9354)
  • Genomics (12566)
  • Immunology (7687)
  • Microbiology (18979)
  • Molecular Biology (7428)
  • Neuroscience (40944)
  • Paleontology (300)
  • Pathology (1226)
  • Pharmacology and Toxicology (2132)
  • Physiology (3146)
  • Plant Biology (6850)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1893)
  • Systems Biology (5306)
  • Zoology (1087)