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CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization

Ying Feng, Yao Wang, Chen Yang, Ardalan Naseri, Thoru Pederson, Jing Zheng, Xiao Xiao, Shaojie Zhang, Wei Xie, View ORCID ProfileHanhui Ma
doi: https://doi.org/10.1101/2020.02.18.954610
Ying Feng
1School of Life Science and Technology, ShanghaiTech University, Shanghai, China
2School of Biotechnology, East China University of Science and Technology, Shanghai, China
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Yao Wang
3Center for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, THU-PKU Center for Life Sciences, Tsinghua University, Beijing, China
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Chen Yang
1School of Life Science and Technology, ShanghaiTech University, Shanghai, China
4School of Pharmacy, East China University of Science and Technology, Shanghai, China
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Ardalan Naseri
5Department of Computer Science, University of Central Florida, Orlando, FL USA
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Thoru Pederson
6Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA USA
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Jing Zheng
4School of Pharmacy, East China University of Science and Technology, Shanghai, China
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Xiao Xiao
2School of Biotechnology, East China University of Science and Technology, Shanghai, China
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Shaojie Zhang
5Department of Computer Science, University of Central Florida, Orlando, FL USA
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Wei Xie
3Center for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, THU-PKU Center for Life Sciences, Tsinghua University, Beijing, China
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  • For correspondence: xiewei121@tsinghua.edu.cn mahh@shanghaitech.edu.cn
Hanhui Ma
1School of Life Science and Technology, ShanghaiTech University, Shanghai, China
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  • ORCID record for Hanhui Ma
  • For correspondence: xiewei121@tsinghua.edu.cn mahh@shanghaitech.edu.cn
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Abstract

Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1α condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1α condensates possibly driven by phase separation.

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Posted February 19, 2020.
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CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
Ying Feng, Yao Wang, Chen Yang, Ardalan Naseri, Thoru Pederson, Jing Zheng, Xiao Xiao, Shaojie Zhang, Wei Xie, Hanhui Ma
bioRxiv 2020.02.18.954610; doi: https://doi.org/10.1101/2020.02.18.954610
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CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
Ying Feng, Yao Wang, Chen Yang, Ardalan Naseri, Thoru Pederson, Jing Zheng, Xiao Xiao, Shaojie Zhang, Wei Xie, Hanhui Ma
bioRxiv 2020.02.18.954610; doi: https://doi.org/10.1101/2020.02.18.954610

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