Abstract
Human genes are regulated quantitatively, yet the ability to specify the expression level of a native gene accurately and specifically using a defined reagent has remained elusive. Here we show that precise targeting of KRAB repressive domain within regulatory DNA unlocks an endogenous quantitative ‘dial’ that can be engaged at nucleotide resolution to program expression levels across a wide physiologic range, with single-gene specificity and high reproducibly in primary cells.
Competing Interest Statement
M.S.W., C.C., S.G, A.F., F.D.U., and J.A.S. are listed as inventors on patent applications related to the subject matter of the paper; J.P. is an employee of Tune Therapeutics, a for-profit biotechnology company.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted February 11, 2021.
Quantitative dialing of gene expression via precision targeting of KRAB repressor
