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A genetically encoded GRAB sensor for measuring serotonin dynamics in vivo
Jinxia Wan, Wanling Peng, Xuelin Li, Tongrui Qian, Kun Song, Jianzhi Zeng, Fei Deng, Suyu Hao, Jiesi Feng, Peng Zhang, Yajun Zhang, Jing Zou, Sunlei Pan, J. Julius Zhu, Miao Jing, Min Xu, Yulong Li
doi: https://doi.org/10.1101/2020.02.24.962282
Jinxia Wan
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
Wanling Peng
3Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
Xuelin Li
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
Tongrui Qian
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
Kun Song
3Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
Jianzhi Zeng
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
4Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
Fei Deng
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
Suyu Hao
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
Jiesi Feng
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
4Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
Peng Zhang
5Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
Yajun Zhang
5Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
Jing Zou
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
6Department of Biological Sciences, Neurobiology Section, University of Southern California, Los Angeles, CA 90089, USA
Sunlei Pan
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
4Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
J. Julius Zhu
5Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
7School of Medicine, Ningbo University, Ningbo 315010, China
8Donders Institute for Brain, Cognition and Behavior, Radboud University Nijmegen, 6525 EN, Nijmegen, Netherlands
Miao Jing
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
4Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
9Chinese Institute for Brain Research, Beijing 102206, China
Min Xu
3Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
Yulong Li
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China
2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China
4Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
Abstract
Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating a variety of processes in the brain. To directly visualize the dynamics of 5-HT, we developed a genetically encoded GPCR-Activation-Based 5-HT (GRAB5-HT) sensor with high sensitivity, selectivity, and spatiotemporal resolution. GRAB5-HT, detected 5-HT release in multiple physiological and pathological conditions in both flies and mice, and thus provides new insights into the dynamics and mechanisms of 5-HT signaling.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted February 25, 2020.
A genetically encoded GRAB sensor for measuring serotonin dynamics in vivo
Jinxia Wan, Wanling Peng, Xuelin Li, Tongrui Qian, Kun Song, Jianzhi Zeng, Fei Deng, Suyu Hao, Jiesi Feng, Peng Zhang, Yajun Zhang, Jing Zou, Sunlei Pan, J. Julius Zhu, Miao Jing, Min Xu, Yulong Li
bioRxiv 2020.02.24.962282; doi: https://doi.org/10.1101/2020.02.24.962282
A genetically encoded GRAB sensor for measuring serotonin dynamics in vivo
Jinxia Wan, Wanling Peng, Xuelin Li, Tongrui Qian, Kun Song, Jianzhi Zeng, Fei Deng, Suyu Hao, Jiesi Feng, Peng Zhang, Yajun Zhang, Jing Zou, Sunlei Pan, J. Julius Zhu, Miao Jing, Min Xu, Yulong Li
bioRxiv 2020.02.24.962282; doi: https://doi.org/10.1101/2020.02.24.962282
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