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Causes of death in newborn C57BL/6J mice

View ORCID ProfileSara Capas-Peneda, View ORCID ProfileGabriela Munhoz Morello, View ORCID ProfileSofia Lamas, View ORCID ProfileI Anna S Olsson, View ORCID ProfileColin Gilbert
doi: https://doi.org/10.1101/2020.02.25.964551
Sara Capas-Peneda
1Laboratory Animal Science, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, IASO)
2i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, SL, IASO)
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Gabriela Munhoz Morello
1Laboratory Animal Science, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, IASO)
2i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, SL, IASO)
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Sofia Lamas
2i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, SL, IASO)
3Animal Facility, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SL)
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I Anna S Olsson
1Laboratory Animal Science, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, IASO)
2i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal (SCP, GMM, SL, IASO)
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Colin Gilbert
4Babraham Institute, Babraham Hall, Cambridge, CB22 3AT, United Kingdom (CG)
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  • ORCID record for Colin Gilbert
  • For correspondence: colin.gilbert@babraham.ac.uk
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Abstract

Neonatal mortality in wild-type laboratory mice is an overlooked welfare and financial problem in animal facilities around the world. Causes of death are often not reported and its causes remain unknown.

In this study, 324 newborn pups from two breeding colonies of healthy wildtype C57BL/6 mice underwent post-mortem analysis with special focus on obtaining proof of life after birth, evaluation of stomach contents and observation of congenital abnormalities that could compromise survival.

Based on a combination of lung morphology findings, outcome of lung float test, stomach contents and brown adipose tissue colouration, 21.6% of the pups found dead were considered stillbirths. Of the livebirths, only 3.2% were observed to have milk inside the stomach, indicating successful suckling. Congenital abnormalities were diagnosed only in a small fraction of the pups analysed. These results suggest that starvation was the most common cause of death, followed by stillbirth.

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Posted February 26, 2020.
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Causes of death in newborn C57BL/6J mice
Sara Capas-Peneda, Gabriela Munhoz Morello, Sofia Lamas, I Anna S Olsson, Colin Gilbert
bioRxiv 2020.02.25.964551; doi: https://doi.org/10.1101/2020.02.25.964551
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Causes of death in newborn C57BL/6J mice
Sara Capas-Peneda, Gabriela Munhoz Morello, Sofia Lamas, I Anna S Olsson, Colin Gilbert
bioRxiv 2020.02.25.964551; doi: https://doi.org/10.1101/2020.02.25.964551

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