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Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2

Yu Jin Jung, Gun-Soo Park, Jun Hye Moon, Keunbon Ku, Seung-Hwa Beak, Seil Kim, Edmond Changkyun Park, View ORCID ProfileDaeui Park, Jong-Hwan Lee, Cheol Woo Byeon, Joong Jin Lee, Jin-Soo Maeng, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Min Jun Lee, Hong Gi Kim
doi: https://doi.org/10.1101/2020.02.25.964775
Yu Jin Jung
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Gun-Soo Park
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
2Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Republic of Korea
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Jun Hye Moon
3Department of Molecular Diagnostics, WELLS BIO, INC, MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul, Republic of Korea
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Keunbon Ku
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Seung-Hwa Beak
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
4Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea
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Seil Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
5Division of Chemical and Medical Metrology, Center for Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea
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Edmond Changkyun Park
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
6Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea
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Daeui Park
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
4Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea
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  • ORCID record for Daeui Park
Jong-Hwan Lee
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Cheol Woo Byeon
3Department of Molecular Diagnostics, WELLS BIO, INC, MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul, Republic of Korea
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Joong Jin Lee
3Department of Molecular Diagnostics, WELLS BIO, INC, MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul, Republic of Korea
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Jin-Soo Maeng
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
2Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Republic of Korea
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Seong Jun Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Seung Il Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
6Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea
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Bum-Tae Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Min Jun Lee
3Department of Molecular Diagnostics, WELLS BIO, INC, MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul, Republic of Korea
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  • For correspondence: tenork@krict.re.kr mjlee@wellsbio.net
Hong Gi Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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  • For correspondence: tenork@krict.re.kr mjlee@wellsbio.net
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Abstract

Coronavirus disease 2019 (COVID-19) is newly emerging human infectious diseases, which is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within two months of the outbreak, more than 80,000 cases of COVID-19 have been confirmed worldwide. Since the human to human transmission occurred easily and the human infection is rapidly increasing, the sensitive and early diagnosis is essential to prevent the global outbreak. Recently, World Health Organization (WHO) announced various primer and probe sets for SARS-CoV-2 previously developed in China, Germany, Hong Kong, Japan, Thailand, and USA. In this study, we compared the ability to detect SARS-CoV-2 RNA among the seven primer-probe sets for N gene and the three primer-probe sets for Orf1 gene. The result of the comparative analysis represented that the ‘2019-nCoV_N2, N3’ of USA and the ‘ORF1ab’ of China are the most sensitive primer-probe sets for N and Orf1 genes, respectively. Therefore, the appropriate combination from ORF1ab (China), 2019-nCoV_N2, N3 (USA), and NIID_2019-nCOV_N (Japan) sets should be selected for the sensitive and reliable laboratory confirmation of SARS-CoV-2.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 27, 2020.
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Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
Yu Jin Jung, Gun-Soo Park, Jun Hye Moon, Keunbon Ku, Seung-Hwa Beak, Seil Kim, Edmond Changkyun Park, Daeui Park, Jong-Hwan Lee, Cheol Woo Byeon, Joong Jin Lee, Jin-Soo Maeng, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Min Jun Lee, Hong Gi Kim
bioRxiv 2020.02.25.964775; doi: https://doi.org/10.1101/2020.02.25.964775
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Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
Yu Jin Jung, Gun-Soo Park, Jun Hye Moon, Keunbon Ku, Seung-Hwa Beak, Seil Kim, Edmond Changkyun Park, Daeui Park, Jong-Hwan Lee, Cheol Woo Byeon, Joong Jin Lee, Jin-Soo Maeng, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Min Jun Lee, Hong Gi Kim
bioRxiv 2020.02.25.964775; doi: https://doi.org/10.1101/2020.02.25.964775

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