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An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12

View ORCID ProfileCurti Lucia, Pereyra-Bonnet Federico, View ORCID ProfileGimenez Carla Alejandra
doi: https://doi.org/10.1101/2020.02.29.971127
Curti Lucia
1INPA-National Scientific and Technical Research Council (CONICET)- Argentina-University of Buenos Aires, Argentina
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Pereyra-Bonnet Federico
1INPA-National Scientific and Technical Research Council (CONICET)- Argentina-University of Buenos Aires, Argentina
2CASPR Biotech, San Francisco California, USA
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Gimenez Carla Alejandra
2CASPR Biotech, San Francisco California, USA
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  • For correspondence: carla@caspr.bio
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Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has received global attention due to the recent outbreak in China. In this work, we report a CRISPR-Cas12 based diagnostic tool to detect synthetic SARS-CoV-2 RNA sequences in a proof-of-principle evaluation. The test proved to be sensitive, rapid, and potentially portable. These key traits of the CRISPR method are critical for virus detection in regions that lack resources to use the currently available methods.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 02, 2020.
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An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12
Curti Lucia, Pereyra-Bonnet Federico, Gimenez Carla Alejandra
bioRxiv 2020.02.29.971127; doi: https://doi.org/10.1101/2020.02.29.971127
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An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12
Curti Lucia, Pereyra-Bonnet Federico, Gimenez Carla Alejandra
bioRxiv 2020.02.29.971127; doi: https://doi.org/10.1101/2020.02.29.971127

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