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An Improved 4’-Aminomethyltroxsalen-Based DNA Crosslinker for Biotinylation of DNA

Kevin Wielenberg, View ORCID ProfileMiao Wang, Min Yang, Abdullah Ozer, View ORCID ProfileJohn T. Lis, View ORCID ProfileHening Lin
doi: https://doi.org/10.1101/2020.02.29.971317
Kevin Wielenberg
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
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Miao Wang
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
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Min Yang
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
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Abdullah Ozer
2Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
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John T. Lis
2Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
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  • ORCID record for John T. Lis
Hening Lin
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
3Howard Hughes Medical Institute; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
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  • For correspondence: hl379@cornell.edu
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Abstract

Nucleic acid crosslinkers that covalently join commentary strands have applications as both pharmaceuticals and biochemical probes. Psoralen is a popular crosslinker moiety that reacts with double stranded DNA and RNA upon irradiation with long wave UV light. A commercially available compound EZ-Link Psoralen-PEG3-Biotin has been used in many studies to crosslink DNA and double strand RNA for genome-wide investigations. Here we present a novel probe, AP3B, which uses a psoralen derivative, 4’-aminomethyltrioxsalen, to biotinylate nucleic acids. We show that this compound is 8-fold more effective at labeling DNA in cells and several hundred-fold more effective at crosslinking two strands of DNA in vitro than the commercially available compound EZ-Link Psoralen-PEG3-Biotin.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 29, 2020.
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An Improved 4’-Aminomethyltroxsalen-Based DNA Crosslinker for Biotinylation of DNA
Kevin Wielenberg, Miao Wang, Min Yang, Abdullah Ozer, John T. Lis, Hening Lin
bioRxiv 2020.02.29.971317; doi: https://doi.org/10.1101/2020.02.29.971317
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An Improved 4’-Aminomethyltroxsalen-Based DNA Crosslinker for Biotinylation of DNA
Kevin Wielenberg, Miao Wang, Min Yang, Abdullah Ozer, John T. Lis, Hening Lin
bioRxiv 2020.02.29.971317; doi: https://doi.org/10.1101/2020.02.29.971317

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