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Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM

Chuang Liu, Yang Yang, Yuanzhu Gao, Chenguang Shen, Bin Ju, Congcong Liu, Xian Tang, Jinli Wei, Xiaomin Ma, Weilong Liu, Shuman Xu, Yingxia Liu, Jing Yuan, Jing Wu, Zheng Liu, Zheng Zhang, Peiyi Wang, Lei Liu
doi: https://doi.org/10.1101/2020.03.02.972927
Chuang Liu
1Department of Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Yang Yang
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
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Yuanzhu Gao
1Department of Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Chenguang Shen
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
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Bin Ju
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
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Congcong Liu
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
5The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Xian Tang
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
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Jinli Wei
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
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Xiaomin Ma
1Department of Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Weilong Liu
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
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Shuman Xu
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Yingxia Liu
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
4Department for Infectious Diseases, Shenzhen Third People’s Hospital, Shenzhen, Guangdong Province 518112, China
5The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Jing Yuan
4Department for Infectious Diseases, Shenzhen Third People’s Hospital, Shenzhen, Guangdong Province 518112, China
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Jing Wu
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Zheng Liu
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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Zheng Zhang
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
5The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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  • For correspondence: liulei3322@aliyun.com zhangzheng1975@aliyun.com wangpy@sustech.edu.cn
Peiyi Wang
1Department of Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
3Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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  • For correspondence: liulei3322@aliyun.com zhangzheng1975@aliyun.com wangpy@sustech.edu.cn
Lei Liu
2Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen 518112, Guangdong Province, China
5The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, Guangdong Province, China
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  • For correspondence: liulei3322@aliyun.com zhangzheng1975@aliyun.com wangpy@sustech.edu.cn
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Abstract

Since December 2019, the outbreak of Coronavirus Disease 2019 (COVID-19) spread from Wuhan, China to the world, it has caused more than 87,000 diagnosed cases and more than 3,000 deaths globally. To fight against COVID-19, we carried out research for the near native SARS-CoV-2 and report here our preliminary results obtained. The pathogen of the COVID-19, the native SARS-CoV-2, was isolated, amplified and purified in a BSL-3 laboratory. The whole viral architecture of SARS-CoV-2 was examined by transmission electron microscopy (both negative staining and cryo-EM). We observed that the virion particles are roughly spherical or moderately pleiomorphic. Spikes have nail-like shape towards outside with a long body embedded in the envelope. The morphology of virion observed in our result indicates that the S protein of SARS-CoV-2 is in post-fusion state, with S1 disassociated. This state revealed by cryo-EM first time could provide an important information for the identification and relevant clinical research of this new coronavirus.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 05, 2020.
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Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM
Chuang Liu, Yang Yang, Yuanzhu Gao, Chenguang Shen, Bin Ju, Congcong Liu, Xian Tang, Jinli Wei, Xiaomin Ma, Weilong Liu, Shuman Xu, Yingxia Liu, Jing Yuan, Jing Wu, Zheng Liu, Zheng Zhang, Peiyi Wang, Lei Liu
bioRxiv 2020.03.02.972927; doi: https://doi.org/10.1101/2020.03.02.972927
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Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM
Chuang Liu, Yang Yang, Yuanzhu Gao, Chenguang Shen, Bin Ju, Congcong Liu, Xian Tang, Jinli Wei, Xiaomin Ma, Weilong Liu, Shuman Xu, Yingxia Liu, Jing Yuan, Jing Wu, Zheng Liu, Zheng Zhang, Peiyi Wang, Lei Liu
bioRxiv 2020.03.02.972927; doi: https://doi.org/10.1101/2020.03.02.972927

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