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Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient

Jennifer Harcourt, Azaibi Tamin, Xiaoyan Lu, Shifaq Kamili, Senthil Kumar. Sakthivel, Janna Murray, Krista Queen, Ying Tao, View ORCID ProfileClinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, View ORCID ProfileJessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, View ORCID ProfileVineet D. Menachery, View ORCID ProfileNatalie J. Thornburg
doi: https://doi.org/10.1101/2020.03.02.972935
Jennifer Harcourt
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Azaibi Tamin
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Xiaoyan Lu
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Shifaq Kamili
2Eagle Contracting, Atlanta GA, USA
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Senthil Kumar. Sakthivel
2Eagle Contracting, Atlanta GA, USA
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Janna Murray
2Eagle Contracting, Atlanta GA, USA
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Krista Queen
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Ying Tao
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Clinton R. Paden
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Jing Zhang
3IHRC, Atlanta GA, USA
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Yan Li
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Anna Uehara
4ORISE, Oak Ridge TN
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Haibin Wang
3IHRC, Atlanta GA, USA
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Cynthia Goldsmith
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Hannah A. Bullock
5Synergy America, Inc., Atlanta GA, USA
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Lijuan Wang
5Synergy America, Inc., Atlanta GA, USA
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Brett Whitaker
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Brian Lynch
2Eagle Contracting, Atlanta GA, USA
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Rashi Gautam
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Craig Schindewolf
6Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, USA
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Kumari G. Lokugamage
6Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, USA
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Dionna Scharton
7World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston TX, USA
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Jessica A. Plante
7World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston TX, USA
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Divya Mirchandani
6Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, USA
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Steven G. Widen
8Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston TX, USA
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Krishna Narayanan
6Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, USA
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Shinji Makino
6Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, USA
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Thomas G. Ksiazek
7World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston TX, USA
9Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston TX, USA
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Kenneth S. Plante
7World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston TX, USA
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Scott C. Weaver
6Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, USA
7World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston TX, USA
9Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston TX, USA
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Stephen Lindstrom
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Suxiang Tong
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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Vineet D. Menachery
7World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston TX, USA
9Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston TX, USA
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Natalie J. Thornburg
1Centers for Disease Control and Prevention, Atlanta, GA, USA
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  • For correspondence: Nax3@cdc.gov
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ABSTRACT

The etiologic agent of the outbreak of pneumonia in Wuhan China was identified as severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) in January, 2020. The first US patient was diagnosed by the State of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens, and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into two virus repositories, making it broadly available to the public health and research communities. We hope that open access to this important reagent will expedite development of medical countermeasures.

Article Summary Scientists have isolated virus from the first US COVID-19 patient. The isolation and reagents described here will serve as the US reference strain used in research, drug discovery and vaccine testing.

Footnotes

  • Growth kinetics data has been added to this manuscript.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
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Posted March 07, 2020.
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Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient
Jennifer Harcourt, Azaibi Tamin, Xiaoyan Lu, Shifaq Kamili, Senthil Kumar. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery, Natalie J. Thornburg
bioRxiv 2020.03.02.972935; doi: https://doi.org/10.1101/2020.03.02.972935
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Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient
Jennifer Harcourt, Azaibi Tamin, Xiaoyan Lu, Shifaq Kamili, Senthil Kumar. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery, Natalie J. Thornburg
bioRxiv 2020.03.02.972935; doi: https://doi.org/10.1101/2020.03.02.972935

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