Abstract
Amphibian genomes are usually challenging to assemble due to large genome size and high repeat content. The Limnodynastidae is a family of frogs native to Australia, Tasmania and New Guinea. As an anuran lineage that successfully diversified on the Australian continent, it represents an important lineage in the amphibian tree of life but lacks reference genomes. Here we sequenced and annotated the genome of the eastern banjo frog Limnodynastes dumerilii dumerilii to fill this gap. The total length of the genome assembly is 2.38 Gb with a scaffold N50 of 285.9 kb. We identified 1.21 Gb of non-redundant sequences as repetitive elements and annotated 24,548 protein-coding genes in the assembly. BUSCO assessment indicated that more than 94% of the expected vertebrate genes were present in the genome assembly and the gene set. We anticipate that this annotated genome assembly will advance the future study of anuran phylogeny and amphibian genome evolution.
Footnotes
e-mail addresses for all authors: Qiye Li <liqiye{at}genomics.cn>, Qunfei Guo <guoqunfei{at}genomics.cn>, Yang Zhou <zhouyang{at}genomics.cn>, Huishuang Tan < tanhuishuang{at}genomics.cn>, Terry Bertozzi <Terry.Bertozzi{at}samuseum.sa.gov.au>, Yuanzhen Zhu <zhuyuanzhen{at}genomics.cn>, Ji Li <liji1{at}genomics.cn>, Stephen Donnellan <Steve.Donnellan{at}samuseum.sa.gov.au> and Guojie Zhang <guojie.zhang{at}bio.ku.dk>
List of abbreviations
- BUSCO
- Benchmarking Universal Single-Copy Orthologs
- G10K
- Genome 10K
- NCBI
- National Center for Biotechnology Information
- PCR
- Polymerase Chain Reaction
- ORF
- Open Reading Frame
- KEGG
- Kyoto Encyclopedia of Genes and Genomes
- DOGMA
- DOmain-based General Measure for transcriptome and proteome quality Assessment
- CDA
- Conserved Domain Arrangement
- CNGB
- China National GeneBank
- CNSA
- CNGB Sequence Archive