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Asymmetric second-generation genomic incompatibility in interspecific crosses between Ciona robusta and Ciona intestinalis

Naoyuki Ohta, Nicole Kaplan, James Tyler Ng, Basile Jules Gravez, View ORCID ProfileLionel Christiaen
doi: https://doi.org/10.1101/2020.03.04.976837
Naoyuki Ohta
Center for Developmental Genetics, Department of Biology New York University, New York, NY, USA
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  • For correspondence: no22@nyu.edu lc121@nyu.edu
Nicole Kaplan
Center for Developmental Genetics, Department of Biology New York University, New York, NY, USA
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James Tyler Ng
Center for Developmental Genetics, Department of Biology New York University, New York, NY, USA
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Basile Jules Gravez
Center for Developmental Genetics, Department of Biology New York University, New York, NY, USA
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Lionel Christiaen
Center for Developmental Genetics, Department of Biology New York University, New York, NY, USA
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  • ORCID record for Lionel Christiaen
  • For correspondence: no22@nyu.edu lc121@nyu.edu
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Abstract

Reproductive isolation is central to speciation, but interspecific crosses between two closely related species can produce viable and fertile hybrids. Two different species in the tunicate genus Ciona, Ciona robusta and Ciona intestinalis can produce hybrids. However, wild sympatric populations display limited gene flow, suggesting the existence of obstacles to interspecific reproduction that remain unknown. Here, we took advantage of a closed inland culture system to cross C. robusta with C. intestinalis and established F1 and F2 hybrids. We monitored post-embryonic development, survival, and sexual maturation to further probe the physiological mechanisms underlying reproductive isolation. Partial viability of first and second generation hybrids indicated that both pre- and postzygotic mechanisms contributed to genomic incompatibilities in hybrids. Asymmetrical second generation inviability and infertility suggested that interspecific genomic incompatibilities involved interactions between the maternal, zygotic and mitochondrial genomes during development. This study paves the way to quantitative genetic approaches to study the mechanisms underlying genomic incompatibilities and other complex traits in the genome-enabled Ciona model.

Footnotes

  • Nomenclature for hybrids and backcrossed animals, corrections for the proper use of genetic terms, clarification of the reproductive mode of Ciona. The authors are grateful to Pr. Matthew Rockman for invaluable feedbacks in the previous version of this manuscript.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 20, 2020.
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Asymmetric second-generation genomic incompatibility in interspecific crosses between Ciona robusta and Ciona intestinalis
Naoyuki Ohta, Nicole Kaplan, James Tyler Ng, Basile Jules Gravez, Lionel Christiaen
bioRxiv 2020.03.04.976837; doi: https://doi.org/10.1101/2020.03.04.976837
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Asymmetric second-generation genomic incompatibility in interspecific crosses between Ciona robusta and Ciona intestinalis
Naoyuki Ohta, Nicole Kaplan, James Tyler Ng, Basile Jules Gravez, Lionel Christiaen
bioRxiv 2020.03.04.976837; doi: https://doi.org/10.1101/2020.03.04.976837

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