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Direct RNA sequencing and early evolution of SARS-CoV-2
George Taiaroa, Daniel Rawlinson, Leo Featherstone, Miranda Pitt, Leon Caly, Julian Druce, Damian Purcell, Leigh Harty, Thomas Tran, Jason Roberts, Mike Catton, Deborah Williamson, Lachlan Coin, Sebastian Duchene
doi: https://doi.org/10.1101/2020.03.05.976167
George Taiaroa
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
2Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Victoria, Australia
Daniel Rawlinson
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Leo Featherstone
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Miranda Pitt
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Leon Caly
2Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Victoria, Australia
Julian Druce
2Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Victoria, Australia
Damian Purcell
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Leigh Harty
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Thomas Tran
2Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Victoria, Australia
Jason Roberts
2Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Victoria, Australia
Mike Catton
2Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Victoria, Australia
Deborah Williamson
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
3Department of Microbiology, Royal Melbourne Hospital, Victoria, Australia
Lachlan Coin
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Sebastian Duchene
1Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia
Abstract
The rapid sharing of sequence information as seen throughout the current SARS-CoV-2 epidemic, represents an inflection point for genomic epidemiology. Here we describe aspects of coronavirus evolutionary genetics revealed from these data, and provide the first direct RNA sequence of SARS-CoV-2, detailing coronaviral subgenome-length mRNA architecture.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted March 07, 2020.
Direct RNA sequencing and early evolution of SARS-CoV-2
George Taiaroa, Daniel Rawlinson, Leo Featherstone, Miranda Pitt, Leon Caly, Julian Druce, Damian Purcell, Leigh Harty, Thomas Tran, Jason Roberts, Mike Catton, Deborah Williamson, Lachlan Coin, Sebastian Duchene
bioRxiv 2020.03.05.976167; doi: https://doi.org/10.1101/2020.03.05.976167
Direct RNA sequencing and early evolution of SARS-CoV-2
George Taiaroa, Daniel Rawlinson, Leo Featherstone, Miranda Pitt, Leon Caly, Julian Druce, Damian Purcell, Leigh Harty, Thomas Tran, Jason Roberts, Mike Catton, Deborah Williamson, Lachlan Coin, Sebastian Duchene
bioRxiv 2020.03.05.976167; doi: https://doi.org/10.1101/2020.03.05.976167
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