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Improved stability of an engineered function using adapted bacterial strains

View ORCID ProfileDrew S. Tack, Peter D. Tonner, Elena Musteata, Vanya Paralanov, View ORCID ProfileDavid Ross
doi: https://doi.org/10.1101/2020.03.05.979385
Drew S. Tack
1Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
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  • For correspondence: drew.tack@nist.gov
Peter D. Tonner
2Statistical Engineering Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
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Elena Musteata
1Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
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Vanya Paralanov
1Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
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David Ross
1Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
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Abstract

Engineering useful functions into cells is one of the primary goals of synthetic biology. However, engineering novel functions that remain stable for multiple generations remains a significant challenge. Here we report the importance of host fitness on the stability of an engineered function. We find that the initial fitness of the host cell affects the stability of the engineered function. We demonstrate that adapting a strain to the intended growth condition increases fitness and in turn improves the stability of the engineered function over hundreds of generations. This approach offers a simple and effective method to increase the stability of engineered functions without genomic modification or additional engineering and will be useful in improving the stability of novel, engineered functions in living cells.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
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Posted March 06, 2020.
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Improved stability of an engineered function using adapted bacterial strains
Drew S. Tack, Peter D. Tonner, Elena Musteata, Vanya Paralanov, David Ross
bioRxiv 2020.03.05.979385; doi: https://doi.org/10.1101/2020.03.05.979385
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Improved stability of an engineered function using adapted bacterial strains
Drew S. Tack, Peter D. Tonner, Elena Musteata, Vanya Paralanov, David Ross
bioRxiv 2020.03.05.979385; doi: https://doi.org/10.1101/2020.03.05.979385

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