Abstract
Fluorescence reconstruction microscopy (FRM) describes a class of techniques where transmitted light images are passed into a convolutional neural network which then outputs predicted epifluorescence images. This approach enables many benefits including reduced phototoxicity, freeing up of fluorescence channels, simplified sample preparation, and the ability to re-process legacy data for new insights. However, current FRM benchmarks are abstractions that are difficult to relate to how valuable or trustworthy an FRM prediction is. Here, we relate the conventional benchmarks and demonstrations to practical and familiar cell biology analyses to demonstrate that FRM should be judged in context. We further demonstrate that it performs remarkably well even with lower-magnification microscopy data, as are often collected in high content imaging. Specifically, we present promising results for nuclei, cell-cell junctions, and fine feature reconstruction; provide data-driven experimental design guidelines; and provide the code, sample data, and user manual to enable more widespread adoption of FRM.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Minor re-wording/re-emphasis of figures; addition of complete test dataset.
https://github.com/CohenLabPrinceton/Fluorescence-Reconstruction