Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A high-resolution map of bacteriophage øX174 transcription

View ORCID ProfileDominic Y. Logel, View ORCID ProfilePaul R. Jaschke
doi: https://doi.org/10.1101/2020.03.05.979765
Dominic Y. Logel
Department of Molecular Sciences, Macquarie University, Sydney 2109, New South Wales, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dominic Y. Logel
Paul R. Jaschke
Department of Molecular Sciences, Macquarie University, Sydney 2109, New South Wales, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paul R. Jaschke
  • For correspondence: paul.jaschke@mq.edu.au
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Bacteriophage øX174 is a model virus for studies across the fields of structural biology, genetics, gut microbiome, and synthetic biology, but did not have a high-resolution transcriptome until this work. In this study we used next-generation sequencing to measure the RNA produced from øX174 while infecting its host E. coli C. We broadly confirm the past transcriptome model while revealing several interesting deviations from previous knowledge. Additionally, we measure the strength of canonical øX174 promoters and terminators and discover both a putative new promoter that may be activated by heat shock sigma factors, as well as rediscover a controversial Rho-dependent terminator. We also provide evidence for the first antisense transcription observed in the Microviridae family, identify two promoters that may be involved in generating this transcriptional activity, and discuss possible reasons why this RNA may be produced.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 06, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A high-resolution map of bacteriophage øX174 transcription
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A high-resolution map of bacteriophage øX174 transcription
Dominic Y. Logel, Paul R. Jaschke
bioRxiv 2020.03.05.979765; doi: https://doi.org/10.1101/2020.03.05.979765
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A high-resolution map of bacteriophage øX174 transcription
Dominic Y. Logel, Paul R. Jaschke
bioRxiv 2020.03.05.979765; doi: https://doi.org/10.1101/2020.03.05.979765

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4095)
  • Biochemistry (8787)
  • Bioengineering (6493)
  • Bioinformatics (23393)
  • Biophysics (11766)
  • Cancer Biology (9170)
  • Cell Biology (13292)
  • Clinical Trials (138)
  • Developmental Biology (7423)
  • Ecology (11388)
  • Epidemiology (2066)
  • Evolutionary Biology (15121)
  • Genetics (10414)
  • Genomics (14025)
  • Immunology (9150)
  • Microbiology (22110)
  • Molecular Biology (8793)
  • Neuroscience (47455)
  • Paleontology (350)
  • Pathology (1423)
  • Pharmacology and Toxicology (2485)
  • Physiology (3712)
  • Plant Biology (8068)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2216)
  • Systems Biology (6021)
  • Zoology (1251)