Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome

View ORCID ProfileDenis Seyres, View ORCID ProfileAlessandra Cabassi, View ORCID ProfileJohn Lambourne, Frances Burden, Samantha Farrow, Harriet McKinney, Joana Batista, View ORCID ProfileCarly Kempster, View ORCID ProfileMaik Pietzner, Oliver Slingsby, View ORCID ProfileThong Huy Cao, Paulene Quinn, View ORCID ProfileLuca Stefanucci, View ORCID ProfileMatthew C Sims, Karola Rehnstrom, Claire Adams, Amy Frary, Bekir Erguener, Roman Kreuzhuber, Gabriele Mocciaro, Michael Allison, Simona D’Amore, View ORCID ProfileAlbert Koulman, Luigi Grassi, Julian L Griffin, View ORCID ProfileLeong Loke Ng, Adrian Park, View ORCID ProfileDavid B Savage, View ORCID ProfileClaudia Langenberg, View ORCID ProfileChristoph Bock, View ORCID ProfileKate Downes, View ORCID ProfileMichele Vacca, View ORCID ProfilePaul DW Kirk, View ORCID ProfileMattia Frontini
doi: https://doi.org/10.1101/2020.03.06.961805
Denis Seyres
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Denis Seyres
  • For correspondence: ds777@medschl.cam.ac.uk paul.kirk@mrc-bsu.cam.ac.uk mf471@cam.ac.uk
Alessandra Cabassi
4MRC Biostatistics Unit, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alessandra Cabassi
John Lambourne
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for John Lambourne
Frances Burden
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Samantha Farrow
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Harriet McKinney
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joana Batista
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carly Kempster
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carly Kempster
Maik Pietzner
5MRC Epidemiology Unit, University of Cambridge, Cambridge, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Maik Pietzner
Oliver Slingsby
6Department of Cardiovascular Sciences, University of Leicester, Glenfield Hospital, Leicester, United Kingdom
7National Institute for Health Research Leicester Biomedical Research Centre, Glenfield Hospital, Leicester, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thong Huy Cao
6Department of Cardiovascular Sciences, University of Leicester, Glenfield Hospital, Leicester, United Kingdom
7National Institute for Health Research Leicester Biomedical Research Centre, Glenfield Hospital, Leicester, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thong Huy Cao
Paulene Quinn
6Department of Cardiovascular Sciences, University of Leicester, Glenfield Hospital, Leicester, United Kingdom
7National Institute for Health Research Leicester Biomedical Research Centre, Glenfield Hospital, Leicester, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Luca Stefanucci
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
8British Heart Foundation Centre of Excellence,Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Luca Stefanucci
Matthew C Sims
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
9Oxford Haemophilia and Thrombosis Centre, Oxford University Hospitals NHS Foundation Trust, NIHR Oxford Biomedical Research Centre, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Matthew C Sims
Karola Rehnstrom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claire Adams
10Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amy Frary
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bekir Erguener
11Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases, Vienna, Austria. .
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: cbock@cemm.oeaw.ac.at
Roman Kreuzhuber
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
12European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabriele Mocciaro
13Department of Biochemistry and the Cambridge Systems Biology Centre, University of Cambridge, The Sanger Building, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Allison
14Addenbrooke’s Hospital, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Simona D’Amore
14Addenbrooke’s Hospital, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom
15Department of Medicine, Aldo Moro University of Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy
16National Cancer Research Center, IRCCS Istituto Tumori ’Giovanni Paolo II’, Viale Orazio Flacco, 65, 70124 Bari, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Albert Koulman
5MRC Epidemiology Unit, University of Cambridge, Cambridge, UK.
17MRC Elsie Widdowson Laboratory, Cambridge, United Kingdom
18National Institute for Health Research Biomedical Research Centres Core Nutritional Biomarker Laboratory, University of Cambridge, Addenbrooke’s Hospital, Cambridge, United Kingdom
19National Institute for Health Research Biomedical Research Centres Core Metabolomics and Lipidomics Laboratory, University of Cambridge, Addenbrooke’s Hospital, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Albert Koulman
Luigi Grassi
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julian L Griffin
13Department of Biochemistry and the Cambridge Systems Biology Centre, University of Cambridge, The Sanger Building, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leong Loke Ng
6Department of Cardiovascular Sciences, University of Leicester, Glenfield Hospital, Leicester, United Kingdom
7National Institute for Health Research Leicester Biomedical Research Centre, Glenfield Hospital, Leicester, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Leong Loke Ng
Adrian Park
14Addenbrooke’s Hospital, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David B Savage
10Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David B Savage
Claudia Langenberg
5MRC Epidemiology Unit, University of Cambridge, Cambridge, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Claudia Langenberg
Christoph Bock
11Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases, Vienna, Austria. .
20CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria. .
21Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria. .
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christoph Bock
  • For correspondence: cbock@cemm.oeaw.ac.at cbock@cemm.oeaw.ac.at cbock@cemm.oeaw.ac.at
Kate Downes
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
2Department of Haematology, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
22East Midlands and East of England Genomic Laboratory Hub, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kate Downes
Michele Vacca
10Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK
13Department of Biochemistry and the Cambridge Systems Biology Centre, University of Cambridge, The Sanger Building, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michele Vacca
Paul DW Kirk
4MRC Biostatistics Unit, University of Cambridge, Cambridge, Cambridge Biomedical Campus, United Kingdom
23Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge CB2 0AW, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paul DW Kirk
  • For correspondence: ds777@medschl.cam.ac.uk paul.kirk@mrc-bsu.cam.ac.uk mf471@cam.ac.uk
Mattia Frontini
1National Institute for Health Research BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom
3NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom
8British Heart Foundation Centre of Excellence,Cambridge Biomedical Campus, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mattia Frontini
  • For correspondence: ds777@medschl.cam.ac.uk paul.kirk@mrc-bsu.cam.ac.uk mf471@cam.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Providing a molecular characterisation of cardiometabolic syndrome (CMS) could improve our understanding of its pathogenesis and pathophysiology, and provide a step toward the development of better treatments. To this end, we performed a deep phenotyping analysis of 185 blood donors, 10 obese, and 10 lipodystrophy patients. We analysed transcriptomes and epigenomes of monocytes, neutrophils, macrophages and platelets. Additionally, plasma metabolites including lipids and biochemistry measurements were quantified.

Multi-omics integration of this data allowed us to identify combinations of features related to patient status and to order the donor population according to their molecular similarity to patients. We also performed differential analyses on epigenomic, transcriptomic and plasma proteomic data collected from obese individuals before and six months after bariatric surgery. These analyses revealed a pattern of abnormal activation of immune cells in obese individuals and lipodystrophy patients, which was partially reverted six months after bariatric surgery.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 06, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
Denis Seyres, Alessandra Cabassi, John Lambourne, Frances Burden, Samantha Farrow, Harriet McKinney, Joana Batista, Carly Kempster, Maik Pietzner, Oliver Slingsby, Thong Huy Cao, Paulene Quinn, Luca Stefanucci, Matthew C Sims, Karola Rehnstrom, Claire Adams, Amy Frary, Bekir Erguener, Roman Kreuzhuber, Gabriele Mocciaro, Michael Allison, Simona D’Amore, Albert Koulman, Luigi Grassi, Julian L Griffin, Leong Loke Ng, Adrian Park, David B Savage, Claudia Langenberg, Christoph Bock, Kate Downes, Michele Vacca, Paul DW Kirk, Mattia Frontini
bioRxiv 2020.03.06.961805; doi: https://doi.org/10.1101/2020.03.06.961805
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
Denis Seyres, Alessandra Cabassi, John Lambourne, Frances Burden, Samantha Farrow, Harriet McKinney, Joana Batista, Carly Kempster, Maik Pietzner, Oliver Slingsby, Thong Huy Cao, Paulene Quinn, Luca Stefanucci, Matthew C Sims, Karola Rehnstrom, Claire Adams, Amy Frary, Bekir Erguener, Roman Kreuzhuber, Gabriele Mocciaro, Michael Allison, Simona D’Amore, Albert Koulman, Luigi Grassi, Julian L Griffin, Leong Loke Ng, Adrian Park, David B Savage, Claudia Langenberg, Christoph Bock, Kate Downes, Michele Vacca, Paul DW Kirk, Mattia Frontini
bioRxiv 2020.03.06.961805; doi: https://doi.org/10.1101/2020.03.06.961805

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3505)
  • Biochemistry (7346)
  • Bioengineering (5323)
  • Bioinformatics (20260)
  • Biophysics (10016)
  • Cancer Biology (7743)
  • Cell Biology (11300)
  • Clinical Trials (138)
  • Developmental Biology (6437)
  • Ecology (9951)
  • Epidemiology (2065)
  • Evolutionary Biology (13321)
  • Genetics (9361)
  • Genomics (12583)
  • Immunology (7701)
  • Microbiology (19021)
  • Molecular Biology (7441)
  • Neuroscience (41036)
  • Paleontology (300)
  • Pathology (1229)
  • Pharmacology and Toxicology (2137)
  • Physiology (3160)
  • Plant Biology (6860)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1896)
  • Systems Biology (5311)
  • Zoology (1089)