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Force-dependent Piezo1 recruitment to focal adhesions regulates adhesion maturation and turnover specifically in non-transformed cells

View ORCID ProfileMingxi Yao, Ajay Tijore, Charles D Cox, Anushya Hariharan, Guy Tran Van Nhieu, Boris Martinac, Michael Sheetz
doi: https://doi.org/10.1101/2020.03.09.972307
Mingxi Yao
1Mechanobiology Institute, National University of Singapore
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Ajay Tijore
1Mechanobiology Institute, National University of Singapore
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Charles D Cox
2Victor Chang Cardiac Research Institute
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Anushya Hariharan
1Mechanobiology Institute, National University of Singapore
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Guy Tran Van Nhieu
3Collège de France
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Boris Martinac
2Victor Chang Cardiac Research Institute
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Michael Sheetz
1Mechanobiology Institute, National University of Singapore
4Dept of Biological Sciences, National University of Singapore
5Molecular MechanoMedicine Program, Dept. of Biochemistry and Molecular Biology, UTMB, Galveston, TX 77555
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  • For correspondence: misheetz@utmb.edu
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Abstract

Piezo1 is a mechanosensitive Ca2+-permeable channel that has been implicated in a number of mechanosensing processes. It is diffusive on plasma membranes and activated by local membrane tension changes yet recent data suggest that Piezo1 activity is tightly coupled to the integrin-mediated actin cytoskeleton through a poorly understood mechanism. In our studies, we found that Piezo1 stably localizes to RGD matrix adhesions in normal but not in transformed cells. Piezo1 binding requires contractility and triggers local Ca2+ entry during adhesion formation and turnover. In transformed cells, Piezo1 level does not affect adhesion morphology; and there is no detectable Ca2+ influx around adhesions. Based upon these findings, we suggest that Piezo1 is a novel component of integrin-based adhesions in non-transformed cells and contributes to the distinct mechanosensing and Ca2+ signaling in normal vs transformed cells. Concentration of Piezo1 at adhesions is a key factor underlying Piezo1’s physiological functions.

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Posted March 09, 2020.
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Force-dependent Piezo1 recruitment to focal adhesions regulates adhesion maturation and turnover specifically in non-transformed cells
Mingxi Yao, Ajay Tijore, Charles D Cox, Anushya Hariharan, Guy Tran Van Nhieu, Boris Martinac, Michael Sheetz
bioRxiv 2020.03.09.972307; doi: https://doi.org/10.1101/2020.03.09.972307
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Force-dependent Piezo1 recruitment to focal adhesions regulates adhesion maturation and turnover specifically in non-transformed cells
Mingxi Yao, Ajay Tijore, Charles D Cox, Anushya Hariharan, Guy Tran Van Nhieu, Boris Martinac, Michael Sheetz
bioRxiv 2020.03.09.972307; doi: https://doi.org/10.1101/2020.03.09.972307

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