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Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2

Gun-Soo Park, Keunbon Ku, Seung-Hwa Baek, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Jin-Soo Maeng
doi: https://doi.org/10.1101/2020.03.09.983064
Gun-Soo Park
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
2Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Republic of Korea
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  • For correspondence: pcdhmk@krict.re.kr maengjs@krict.re.kr
Keunbon Ku
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Seung-Hwa Baek
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
3Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea
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Seong Jun Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Seung Il Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
4Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea
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Bum-Tae Kim
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
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Jin-Soo Maeng
1Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
2Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Republic of Korea
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  • For correspondence: pcdhmk@krict.re.kr maengjs@krict.re.kr
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Abstract

Epidemics of Coronavirus Disease 2019 (COVID-19) now have more than 100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by RT-qPCR methods, but the capacity of RT-qPCR methods is limited by its requirement of high-level facilities and instruments. Here, we developed and evaluated RT-LAMP assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human Coronaviruses was not observed. We also adapted a colorimetric detection method for our RT-LAMP assay so that the tests potentially performed in higher throughput.

Footnotes

  • Erratum fixed in an author's name.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 24, 2020.
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Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
Gun-Soo Park, Keunbon Ku, Seung-Hwa Baek, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Jin-Soo Maeng
bioRxiv 2020.03.09.983064; doi: https://doi.org/10.1101/2020.03.09.983064
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Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
Gun-Soo Park, Keunbon Ku, Seung-Hwa Baek, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Jin-Soo Maeng
bioRxiv 2020.03.09.983064; doi: https://doi.org/10.1101/2020.03.09.983064

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