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A pancreas specific Ptf1a-driven Cre mouse line causes paternally transmitted germline recombination

View ORCID ProfileDerya Kabacaoglu, Marina Lesina, View ORCID ProfileHana Algül
doi: https://doi.org/10.1101/2020.03.13.989178
Derya Kabacaoglu
1TUM, 81675 Munich, Germany
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Marina Lesina
1TUM, 81675 Munich, Germany
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Hana Algül
1TUM, 81675 Munich, Germany
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  • For correspondence: hana.alguel@mri.tum.de
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Abstract

Cre-loxP recombination system is a commonly used tool to achieve site-specific genetic manipulation in genome. For multiple Cre driver mouse lines, parental transmissions of recombined flox alleles are reported. Ptf1a-driven Cre lines are widely used to achieve genetic manipulation in a pancreas specific manner. Herein, we report germline recombination in breedings when Cre allele is retained paternally in Ptf1atm1(cre)Hnak. The germline recombination frequency changed depending on the target allele. Therefore, unless the reporter allele is on the target gene, the reporter activity is to be validated. Overall, we highlight that all Ptf1a-driven Cre mouse lines should be genotyped for possible germline recombination and we advise the maternal transmission of the Cre to progeny.

Footnotes

  • Additional Information: This project is funded by grants AL1174/5-2, DI2299/1-1 and AL1174/6-1 from Deutsche Forschungsgemeinschaft.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 18, 2020.
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A pancreas specific Ptf1a-driven Cre mouse line causes paternally transmitted germline recombination
Derya Kabacaoglu, Marina Lesina, Hana Algül
bioRxiv 2020.03.13.989178; doi: https://doi.org/10.1101/2020.03.13.989178
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A pancreas specific Ptf1a-driven Cre mouse line causes paternally transmitted germline recombination
Derya Kabacaoglu, Marina Lesina, Hana Algül
bioRxiv 2020.03.13.989178; doi: https://doi.org/10.1101/2020.03.13.989178

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