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RBD mutations from circulating SARS-CoV-2 strains enhance the structure stability and infectivity of the spike protein

Junxian Ou, Zhonghua Zhou, Jing Zhang, Wendong Lan, Shan Zhao, Jianguo Wu, Donald Seto, Gong Zhang, Qiwei Zhang
doi: https://doi.org/10.1101/2020.03.15.991844
Junxian Ou
1Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China
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Zhonghua Zhou
2Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
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Jing Zhang
3Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, Guangdong 510632, China
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Wendong Lan
1Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China
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Shan Zhao
1Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China
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Jianguo Wu
3Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, Guangdong 510632, China
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Donald Seto
4Bioinformatics and Computational Biology Program, School of Systems Biology, George Mason University, Manassas, VA 20110, USA
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Gong Zhang
2Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
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  • For correspondence: zhangqw@smu.edu.cn zhanggong@jnu.edu.cn
Qiwei Zhang
1Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China
3Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, Guangdong 510632, China
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  • For correspondence: zhangqw@smu.edu.cn zhanggong@jnu.edu.cn
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Abstract

A novel zoonotic coronavirus SARS-CoV-2 is associated with the current global pandemic of Coronavirus Disease 2019 (COVID-19). Bats and pangolins are suspected as the reservoir and the intermediate host. The receptor binding domain (RBD) of the SARS-CoV-2 S protein plays the key role in the tight binding to human ACE2 for viral entry. In this study, we analyzed the worldwide RBD mutations and found 10 mutants under high positive selection pressure during the spread. The equilibrium dissociation constant (KD) of three RBD mutants emerging in Wuhan, Shenzhen, Hong Kong and France were two orders of magnitude lower than the prototype Wuhan-Hu-1 strain due to the stabilization of the beta-sheet scaffold of the RBD. This indicated that the mutated viruses have evolved to acquire remarkably increased infectivity. Five France isolates and one Hong Kong isolate shared the same RBD mutation enhancing the binding affinity, which suggested that they may have originated as a novel sub-lineage. The KD values for the bat and the pangolin SARS-like CoV RBDs indicated that it would be difficult and unlikely for this bat SARS-like CoV to infect humans; however, the pangolin CoV is potentially infectious to humans with respect to its RBD. These analyses of critical mutations of the RBD provide further insights into the molecular evolution of SARS-CoV-2, presumably while under selection pressure. This enhancement of the SARS-CoV-2 binding affinity to its host receptor ACE2 reveals a higher risk of more severe infections during a sustained pandemic of COVID-19 if no effective precautions are implemented.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 17, 2020.
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RBD mutations from circulating SARS-CoV-2 strains enhance the structure stability and infectivity of the spike protein
Junxian Ou, Zhonghua Zhou, Jing Zhang, Wendong Lan, Shan Zhao, Jianguo Wu, Donald Seto, Gong Zhang, Qiwei Zhang
bioRxiv 2020.03.15.991844; doi: https://doi.org/10.1101/2020.03.15.991844
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RBD mutations from circulating SARS-CoV-2 strains enhance the structure stability and infectivity of the spike protein
Junxian Ou, Zhonghua Zhou, Jing Zhang, Wendong Lan, Shan Zhao, Jianguo Wu, Donald Seto, Gong Zhang, Qiwei Zhang
bioRxiv 2020.03.15.991844; doi: https://doi.org/10.1101/2020.03.15.991844

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