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Gsx2 is involved in specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neuronal progenitors in zebrafish

Tsubasa Itoh, Miki Takeuchi, Marina Sakagami, Kazuhide Asakawa, Koichi Kawakami, Takashi Shimizu, View ORCID ProfileMasahiko Hibi
doi: https://doi.org/10.1101/2020.03.16.993402
Tsubasa Itoh
1Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8602, Japan
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Miki Takeuchi
2Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8601, Japan
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Marina Sakagami
1Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8602, Japan
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Kazuhide Asakawa
3Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
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Koichi Kawakami
3Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
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Takashi Shimizu
1Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8602, Japan
2Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8601, Japan
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Masahiko Hibi
1Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8602, Japan
2Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya, Aichi 464-8601, Japan
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  • ORCID record for Masahiko Hibi
  • For correspondence: hibi@bio.nagoya-u.ac.jp
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ABSTRACT

Neurons in the inferior olivary nuclei (IO neurons) send climbing fibers to Purkinje cells to elicit functions of the cerebellum. IO neurons and Purkinje cells are derived from neural progenitors expressing the proneural gene ptf1a. In this study, we found that the homeobox gene gsx2 was co-expressed with ptf1a in IO progenitors in zebrafish. Both gsx2 and ptf1a zebrafish mutants showed a strong reduction or loss of IO neurons. The expression of ptf1a was not affected in gsx2 mutants and vice versa. In IO progenitors, the ptf1a mutation increased apoptosis whereas the gsx2 mutation did not, suggesting that ptf1a and gsx2 are independently regulated and have distinct roles. The fibroblast growth factors (Fgf) 3/8a and retinoic acid signals negatively and positively, respectively, regulated gsx2 expression and thereby the development of IO neurons. mafba and hox genes are at least partly involved in the Fgf- and retinoic acid-dependent regulation of IO neuronal development. Our results indicate that gsx2 mediates the rostro-caudal positional signals to specify the identity of IO neurons from ptf1a-expressing neural progenitors.

Summary The homeobox gene gsx2 mediates rostro-caudal positional signaling to specify the identify of neurons in the inferior olivary nuclei from neural progenitors expressing the proneural gene ptf1a.

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Posted March 17, 2020.
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Gsx2 is involved in specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neuronal progenitors in zebrafish
Tsubasa Itoh, Miki Takeuchi, Marina Sakagami, Kazuhide Asakawa, Koichi Kawakami, Takashi Shimizu, Masahiko Hibi
bioRxiv 2020.03.16.993402; doi: https://doi.org/10.1101/2020.03.16.993402
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Gsx2 is involved in specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neuronal progenitors in zebrafish
Tsubasa Itoh, Miki Takeuchi, Marina Sakagami, Kazuhide Asakawa, Koichi Kawakami, Takashi Shimizu, Masahiko Hibi
bioRxiv 2020.03.16.993402; doi: https://doi.org/10.1101/2020.03.16.993402

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