Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2

Erik Procko
doi: https://doi.org/10.1101/2020.03.16.994236
Erik Procko
Department of Biochemistry, University of Illinois, Urbana IL 61801 Email:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: coronavirus-research@illinois.edu coronavirus-research@illinois.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

SUMMARY

The rapid and escalating spread of SARS coronavirus 2 (SARS-CoV-2) poses an immediate public health emergency, and no approved therapeutics or vaccines are currently available. The viral spike protein S binds ACE2 on host cells to initiate molecular events that release the viral genome intracellularly. Soluble ACE2 inhibits entry of both SARS and SARS-2 coronaviruses by acting as a decoy for S binding sites, and is a candidate for therapeutic and prophylactic development. Using deep mutagenesis, variants of ACE2 are identified with increased binding to the receptor binding domain of S at a cell surface. Mutations are found across the interface and also at buried sites where they are predicted to enhance folding and presentation of the interaction epitope. The N90-glycan on ACE2 hinders association. The mutational landscape offers a blueprint for engineering high affinity ACE2 receptors to meet this unprecedented challenge.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 17, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2
Erik Procko
bioRxiv 2020.03.16.994236; doi: https://doi.org/10.1101/2020.03.16.994236
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2
Erik Procko
bioRxiv 2020.03.16.994236; doi: https://doi.org/10.1101/2020.03.16.994236

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (4381)
  • Biochemistry (9590)
  • Bioengineering (7090)
  • Bioinformatics (24856)
  • Biophysics (12600)
  • Cancer Biology (9955)
  • Cell Biology (14348)
  • Clinical Trials (138)
  • Developmental Biology (7946)
  • Ecology (12105)
  • Epidemiology (2067)
  • Evolutionary Biology (15985)
  • Genetics (10924)
  • Genomics (14738)
  • Immunology (9869)
  • Microbiology (23657)
  • Molecular Biology (9484)
  • Neuroscience (50847)
  • Paleontology (369)
  • Pathology (1539)
  • Pharmacology and Toxicology (2681)
  • Physiology (4013)
  • Plant Biology (8657)
  • Scientific Communication and Education (1508)
  • Synthetic Biology (2393)
  • Systems Biology (6433)
  • Zoology (1346)