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Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue

Astrid M. Alsema, Qiong Jiang, Laura Kracht, Emma Gerrits, Marissa L. Dubbelaar, Anneke Miedema, Nieske Brouwer, Maya Woodbury, Astrid Wachter, Hualin S. Xi, Thomas Möller, Knut P. Biber, Susanne M. Kooistra, Erik W.G.M Boddeke, Bart J.L. Eggen
doi: https://doi.org/10.1101/2020.03.18.995332
Astrid M. Alsema
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Qiong Jiang
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Laura Kracht
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Emma Gerrits
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Marissa L. Dubbelaar
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Anneke Miedema
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Nieske Brouwer
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Maya Woodbury
3Foundational Neuroscience Center, AbbVie Inc., Cambridge, Massachusetts, United States of America
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Astrid Wachter
4Neuroscience Discovery, AbbVie Deutschland GmbH & Co. KG, Ludwigshafen, Germany
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Hualin S. Xi
3Foundational Neuroscience Center, AbbVie Inc., Cambridge, Massachusetts, United States of America
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Thomas Möller
3Foundational Neuroscience Center, AbbVie Inc., Cambridge, Massachusetts, United States of America
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Knut P. Biber
4Neuroscience Discovery, AbbVie Deutschland GmbH & Co. KG, Ludwigshafen, Germany
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Susanne M. Kooistra
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Erik W.G.M Boddeke
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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Bart J.L. Eggen
1Department of Biomedical Sciences of Cells & Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, the Netherlands
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  • For correspondence: b.j.l.eggen@umcg.nl
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Abstract

Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease. Here, we investigated microglia transcriptomes at bulk and single cell level in non-demented elderly and AD donors using acute human post-mortem cortical brain samples. We identified 9 human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.

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Posted March 19, 2020.
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Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue
Astrid M. Alsema, Qiong Jiang, Laura Kracht, Emma Gerrits, Marissa L. Dubbelaar, Anneke Miedema, Nieske Brouwer, Maya Woodbury, Astrid Wachter, Hualin S. Xi, Thomas Möller, Knut P. Biber, Susanne M. Kooistra, Erik W.G.M Boddeke, Bart J.L. Eggen
bioRxiv 2020.03.18.995332; doi: https://doi.org/10.1101/2020.03.18.995332
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Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue
Astrid M. Alsema, Qiong Jiang, Laura Kracht, Emma Gerrits, Marissa L. Dubbelaar, Anneke Miedema, Nieske Brouwer, Maya Woodbury, Astrid Wachter, Hualin S. Xi, Thomas Möller, Knut P. Biber, Susanne M. Kooistra, Erik W.G.M Boddeke, Bart J.L. Eggen
bioRxiv 2020.03.18.995332; doi: https://doi.org/10.1101/2020.03.18.995332

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