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The Irish potato famine pathogen subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface

View ORCID ProfilePooja Pandey, View ORCID ProfileAlexandre Y Leary, Yasin Tümtas, Zachary Savage, Bayantes Dagvadorj, Emily Tan, Virendrasinh Khandare, View ORCID ProfileCian Duggan, Temur Yusunov, Mathias Madalinski, Federico Gabriel Mirkin, Sebastian Schornack, Yasin Dagdas, Sophien Kamoun, Tolga O. Bozkurt
doi: https://doi.org/10.1101/2020.03.20.000117
Pooja Pandey
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Alexandre Y Leary
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Yasin Tümtas
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Zachary Savage
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Bayantes Dagvadorj
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Emily Tan
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Virendrasinh Khandare
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Cian Duggan
1Imperial College London, Exhibition Road SW7 2AZ London UK
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Temur Yusunov
2Sainsbury Laboratory Cambridge University (SLCU), Cambridge, UK
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Mathias Madalinski
3Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Federico Gabriel Mirkin
4INGEBI-CONICET, Ciudad Autonoma de Buenos Aires, Argentina
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Sebastian Schornack
2Sainsbury Laboratory Cambridge University (SLCU), Cambridge, UK
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Yasin Dagdas
3Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
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Sophien Kamoun
5The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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Tolga O. Bozkurt
1Imperial College London, Exhibition Road SW7 2AZ London UK
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  • For correspondence: o.bozkurt@imperial.ac.uk
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Summary

Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How and why adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phythophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway, while antagonizing antimicrobial autophagy. Here we show that PexRD54 induces autophagosome formation by bridging small GTPase Rab8a-decorated vesicles with autophagic compartments labelled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing that specific trafficking pathways underpin selective autophagy. We discovered that Rab8a contributes to basal immunity against P. infestans, but PexRD54 diverts a sub-population of Rab8a vesicles to lipid droplets that associate with autophagosomes. These are then diverted towards pathogen feeding structures that are accommodated within the host cells. We propose that PexRD54 mimics starvation-induced autophagy by channeling host endomembrane trafficking towards the pathogen interface possibly to acquire nutrients. This work reveals that effectors can interconnect independent host compartments to stimulate complex cellular processes that benefit the pathogen.

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Posted March 21, 2020.
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The Irish potato famine pathogen subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
Pooja Pandey, Alexandre Y Leary, Yasin Tümtas, Zachary Savage, Bayantes Dagvadorj, Emily Tan, Virendrasinh Khandare, Cian Duggan, Temur Yusunov, Mathias Madalinski, Federico Gabriel Mirkin, Sebastian Schornack, Yasin Dagdas, Sophien Kamoun, Tolga O. Bozkurt
bioRxiv 2020.03.20.000117; doi: https://doi.org/10.1101/2020.03.20.000117
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The Irish potato famine pathogen subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
Pooja Pandey, Alexandre Y Leary, Yasin Tümtas, Zachary Savage, Bayantes Dagvadorj, Emily Tan, Virendrasinh Khandare, Cian Duggan, Temur Yusunov, Mathias Madalinski, Federico Gabriel Mirkin, Sebastian Schornack, Yasin Dagdas, Sophien Kamoun, Tolga O. Bozkurt
bioRxiv 2020.03.20.000117; doi: https://doi.org/10.1101/2020.03.20.000117

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