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RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWAB USING QIAGEN RNEASY KITS OR DIRECTLY VIA OMISSION OF AN RNA EXTRACTION STEP

View ORCID ProfileEmily A. Bruce, Scott Tighe, Jessica J. Hoffman, Pheobe Laaguiby, Diana L. Gerrard, View ORCID ProfileSean A. Diehl, View ORCID ProfileDebra G. B. Leonard, Christopher D. Huston, Beth D. Kirkpatrick, Jessica W. Crothers, View ORCID ProfileJulie Dragon, View ORCID ProfileJason Botten
doi: https://doi.org/10.1101/2020.03.20.001008
Emily A. Bruce
1Department of Medicine, Division of Immmunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
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  • ORCID record for Emily A. Bruce
  • For correspondence: Emily.bruce@med.uvm.edu Jason.botten@med.uvm.edu
Scott Tighe
2Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
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Jessica J. Hoffman
2Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
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Pheobe Laaguiby
2Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
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Diana L. Gerrard
3Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington VT, 05401, USA
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Sean A. Diehl
4Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
5Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA
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  • ORCID record for Sean A. Diehl
Debra G. B. Leonard
6Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA
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  • ORCID record for Debra G. B. Leonard
Christopher D. Huston
4Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
7Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA
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Beth D. Kirkpatrick
4Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
5Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA
7Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA
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Jessica W. Crothers
6Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA
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Julie Dragon
2Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
4Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
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Jason Botten
1Department of Medicine, Division of Immmunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
4Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
5Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA
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  • ORCID record for Jason Botten
  • For correspondence: Emily.bruce@med.uvm.edu Jason.botten@med.uvm.edu
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Article Information

doi 
https://doi.org/10.1101/2020.03.20.001008
History 
  • March 21, 2020.

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  • You are currently viewing Version 1 of this article (March 21, 2020 - 15:45).
  • View Version 2, the most recent version of this article.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Emily A. Bruce1,*,
  2. Scott Tighe2,
  3. Jessica J. Hoffman2,
  4. Pheobe Laaguiby2,
  5. Diana L. Gerrard3,
  6. Sean A. Diehl4,5,
  7. Debra G. B. Leonard6,
  8. Christopher D. Huston4,7,
  9. Beth D. Kirkpatrick4,5,7,
  10. Jessica W. Crothers6,
  11. Julie Dragon2,4 and
  12. Jason Botten1,4,5,*
  1. 1Department of Medicine, Division of Immmunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
  2. 2Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
  3. 3Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington VT, 05401, USA
  4. 4Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA
  5. 5Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA
  6. 6Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA
  7. 7Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA
  1. ↵*To whom correspondence should be addressed: Emily.bruce{at}med.uvm.edu (EB), Jason.botten{at}med.uvm.edu (JB)
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RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWAB USING QIAGEN RNEASY KITS OR DIRECTLY VIA OMISSION OF AN RNA EXTRACTION STEP
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RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWAB USING QIAGEN RNEASY KITS OR DIRECTLY VIA OMISSION OF AN RNA EXTRACTION STEP
Emily A. Bruce, Scott Tighe, Jessica J. Hoffman, Pheobe Laaguiby, Diana L. Gerrard, Sean A. Diehl, Debra G. B. Leonard, Christopher D. Huston, Beth D. Kirkpatrick, Jessica W. Crothers, Julie Dragon, Jason Botten
bioRxiv 2020.03.20.001008; doi: https://doi.org/10.1101/2020.03.20.001008
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RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWAB USING QIAGEN RNEASY KITS OR DIRECTLY VIA OMISSION OF AN RNA EXTRACTION STEP
Emily A. Bruce, Scott Tighe, Jessica J. Hoffman, Pheobe Laaguiby, Diana L. Gerrard, Sean A. Diehl, Debra G. B. Leonard, Christopher D. Huston, Beth D. Kirkpatrick, Jessica W. Crothers, Julie Dragon, Jason Botten
bioRxiv 2020.03.20.001008; doi: https://doi.org/10.1101/2020.03.20.001008

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